Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Cartpttm1.2(cre/ERT2)Hhz
Name:	CART prepropeptide; targeted mutation 1.2, Herbert Herzog
MGI ID:	MGI:7439824
Synonyms:	CART-, CARTcre
Gene:	Cartpt  Location: Chr13:100034991-100037191 bp, - strand  Genetic Position: Chr13, 52.9 cM, cytoband D1
Alliance:	Cartpttm1.2(cre/ERT2)Hhz page

Germline Transmission:	Earliest citation of germline transmission: J:333645
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	Not Specified

Allele Type:    Targeted (Inducible, Null/knockout, Recombinase, Reporter) Inducer:    tamoxifen Mutations:    Insertion, Intragenic deletion   Cartpttm1.2(cre/ERT2)Hhz expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Cartpt (MGI:1351330)   Mutation details: The targeting vector was designed to replace the CART coding exons with the mouse Cartpt cDNA fused to the original CART gene at the initiation codon, which is followed by the insertion of a flippase (Flp) recombinase-recombination target (FRT)-flanked neomycin selection cassette. A cassette carrying the tamoxifen-inducible Cre-recombinase gene followed by an internal ribosome entry site (IRES) that drives the expression of the mCherry red fluorescence reporter gene was inserted. Chimeric offspring were bred with a germline Flp-recombinase mouse line to remove the neomycin cassette. LoxP sites were placed immediately upstream of the initiation codon of the CART and Cre genes respectively, which allows the replacement of the CART gene by Cre. Homozygous mice were then crossed with mice that expressed Cre specifically in the oocytes, resulting in a rearrangement that combines the inducible Cre gene with the endogenous CART promoter. (J:333645)

Activity:	Tissue activity of this recombinase allele
Driver:	Cartpt (mouse) Summary of all recombinase alleles driven by Cartpt. Comparative matrix view of recombinase activities Alliance mouse Cartpt gene page

In Mice Carrying this Mutation:

17 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cartpt Mutation:	13 strains or lines available

Original:	J:333645 Lau J, et al., Temperature dependence of the control of energy homeostasis requires CART signaling. Neuropeptides. 2016 Oct;59:97-109
All:	2 reference(s)