Antibody:	anti-IFT140
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Structure	Level	Pattern	Image	Note
TS20: limb cartilage condensation	Weak		3B	Expressed in condensed mesenchymal cells of limb buds that give rise to the future cartilage primordium.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: limb long bone	hypertrophic chondrocyte	Strong		3E
TS24: perichondrium	preosteoblast	Strong		3E

Structure	Cell Type	Level	Pattern	Image	Note
TS27: tibia	growth plate cartilage chondrocyte	Strong		4B , 4A
TS27: trabecular bone	osteoblast	Weak	Regionally restricted	4A , 4C	Expressed in the subchondral bone.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate	growth plate cartilage chondrocyte	Present		4E , 4D	Expressed in almost all layers of chondrocytes in the growth plate, arranged in columns.
TS28: trabecular bone	osteoblast	Strong		4F , 4D	Increased expression compared with that of the newborn.
TS28: tibia epiphysis	hypertrophic chondrocyte	Weak	Regionally restricted	4D	Expressed in differentiated chondrocytes near the future secondary ossification center area.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: tibia epiphysis	hypertrophic chondrocyte	Present		4G , 4I	Stained area in the future secondary ossification center area gradually expanded.
TS28: long bone epiphyseal plate proliferative zone	growth plate cartilage chondrocyte	Present		4G , 4H	Stronger expression in the proliferative zone and prehypertrophic zone.
TS28: long bone epiphyseal plate hypertrophic zone	growth plate cartilage chondrocyte	Present		4G , 4J	Weaker expression.
TS28: trabecular bone	osteoblast	Strong		4K , 4G , 4J	Stronger expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: tibia epiphysis	hypertrophic chondrocyte	Present	Regionally restricted	5C , 5A	Expression in newly formed bone tissue in the secondary ossification center increased. Low expression in the remaining chondrocytes.
TS28: long bone epiphyseal plate	growth plate cartilage chondrocyte	Weak		5B , 5A	Decreased expression.
TS28: trabecular bone	osteoblast	Present		5E , 5A , 5D	Decreased expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: tibia epiphysis	hypertrophic chondrocyte	Weak	Regionally restricted	5F , 5H , 5I	Expressed in the secondary ossification center.
TS28: long bone epiphyseal plate	growth plate cartilage chondrocyte	Weak	Regionally restricted	5G , 5F	Expressed a small fraction of chondrocytes.
TS28: trabecular bone	osteoblast	Present	Regionally restricted	5F , 5J	Expressed on the surface of trabecular bone under the growth plate.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Strong		6A 1w	Strong cytoplasmic staining mostly in the prehypertrophic and hypertrophic layers.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Strong		6A 2w	Strong cytoplasmic staining mostly in the prehypertrophic and hypertrophic layers.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Present		6A 4w	Cytoplasmic staining mostly in the prehypertrophic and hypertrophic layers.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Present		6A 8w	Cytoplasmic staining mostly in the prehypertrophic and hypertrophic layers.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: trabecular bone	osteoblast	Strong		6B 1w

Structure	Cell Type	Level	Pattern	Image	Note
TS28: trabecular bone	osteoblast	Strong		6B 2w

Structure	Cell Type	Level	Pattern	Image	Note
TS28: trabecular bone	osteoblast	Present		6B 4w

Structure	Cell Type	Level	Pattern	Image	Note
TS28: trabecular bone	osteoblast	Weak		6B 8w