Id4 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:251222 Sun F, et al., Id4 Marks Spermatogonial Stem Cells in the Mouse Testis. Sci Rep. 2015 Dec 1;5:17594
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7437986
Gene symbol:	Id4
Gene name:	inhibitor of DNA binding 4

Reporter:	tdTomato
	Direct Detection

Results

Specimen 1d - upper: postnatal; Id4^{tm1(cre)Bcgen}/Id4^{tm1(cre)Bcgen} (more

)
Note: Double labeled: red - Id4 reporter; green - Gfra1 (assay MGI:7438153).

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present	Single cells	1d upper tdTomato , 1d upper Merge	Expression is seen in undifferentiated spermatogonia.

Specimen 1d - lower: postnatal; Id4^{tm1(cre)Bcgen}/Id4^{tm1(cre)Bcgen} (more

)
Note: Double labeled: red - Id4 reporter; green - Gfra1 (assay MGI:7438153).

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present	Single cells	1d lower Merge , 1d lower tdTomato	Expression is seen in undifferentiated spermatogonia.

S1a Merge ©

S1a tdTomato ©

Specimen S1a: postnatal; Id4^{tm1(cre)Bcgen}/Id4^{tm1(cre)Bcgen} (more

)
Note: Double labeled: red - Id4 reporter; green - Gfra1 (assay MGI:7438153).

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present	Single cells	S1a tdTomato , S1a Merge

2a Merge ©

2a tdTomato ©

Specimen 2a: postnatal; Id4^{tm1(cre)Bcgen}/Id4^{tm1(cre)Bcgen} (more

)
Note: Double labeled: red - Id4 reporter; green Id4 protein (assay MGI:7438154).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		2a tdTomato , 2a Merge	The reporter and the protein are expressed in the same cells.

S1B Merge ©

S1b tdTomato ©

Specimen S1b: postnatal; Id4^{tm1(cre)Bcgen}/Id4^{tm1(cre)Bcgen} (more

)
Note: Double labeled: red - Id4 reporter; green Id4 protein (assay MGI:7438154).

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		S1b tdTomato , S1B Merge	The reporter and the protein are expressed in the same cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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