Probe:	Gas1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		1C	Broad expression was detected at E9.5.
TS15: otocyst	Present		1C	Broad expression was detected at E9.5.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	1H	Expression was restricted to the dorsal half at E10.5.
TS17: otocyst	Present	Regionally restricted	1H	Expression was restricted to the dorsal half at E10.5.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Widespread	2D	Broad expression was detected without a clear gradient.
TS20: inner ear mesenchyme	Present	Widespread	2D	Broad expression was detected without a clear gradient.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Widespread	2J	Broad expression was detected without a clear gradient.
TS20: inner ear mesenchyme	Present	Widespread	2J	Broad expression was detected without a clear gradient.
TS20: semicircular canal	Present	Regionally restricted	2J	Expression was detected in the cristae.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Widespread	2P	Broad expression was detected without a clear gradient.
TS20: inner ear mesenchyme	Present	Widespread	2P	Broad expression was detected without a clear gradient.
TS20: utricle	Present	Regionally restricted	2P	Expression was detected in the utricular macula.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Widespread	2V	Broad expression was detected without a clear gradient.
TS20: inner ear mesenchyme	Present	Widespread	2V	Broad expression was detected without a clear gradient.
TS20: cochlea	Present	Regionally restricted	2V	A small region in the lateral cochlea was absent.
TS20: vestibulo-cochlear ganglion	Present		2V

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion	Present	Regionally restricted	3E	Expression was upregulated only at the cochlear level of the cochleovestibular ganglion.

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion	Present	Regionally restricted	3J	Expression was upregulated only at the cochlear level of the cochleovestibular ganglion.

Structure	Level	Pattern	Image	Note
TS23: inner ear	Present	Widespread	4G	Broad expression was detected in the inner ear.
TS23: inner ear vestibular component	Present	Regionally restricted	4G	Expression was detected in sensory organs, but not the roof of the utricular macula.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cochlear duct		Present	Regionally restricted	4H	Expression was detected in the stria vascularis.
TS23: cochlear ganglion	spiral ganglion neuron	Present		4H , 4H'

Structure	Level	Pattern	Image	Note
TS27: inner ear vestibular component	Strong	Regionally restricted	5E'' , 5E , 5E'	Strong expression was detected in the vestibular sensory regions, most likely in supporting cells.
TS27: crista ampullaris	Present		5E , 5E'
TS27: macula of utricle	Present		5E'' , 5E

Structure	Cell Type	Level	Pattern	Image	Note
TS27: external spiral sulcus		Present		5F
TS27: internal spiral sulcus		Present		5F
TS27: vestibular membrane		Present		5F
TS27: cochlea		Present	Regionally restricted	5F	Expression was detected in the mesenchyme adjacent to the developing stria vascularis.
TS27: cochlea	supporting cell of cochlea	Present		5F' , 5F'' , 5F

Structure	Level	Pattern	Image	Note
TS23: middle ear	Present	Regionally restricted	6D	Expression was detected in middle ear joints and annular ligaments.
TS23: stapes	Present	Regionally restricted	6D	Expression was detected in stapedial footplate.

Structure	Level	Pattern	Image	Note
TS23: middle ear	Present	Regionally restricted	6J	Expression was detected in middle ear joints.