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Gene Expression Data
Assay Details
Assay
Reference: J:282974 Lee M, et al., Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol. 2020 Feb 3;219(2)
Assay type: Western blot
MGI Accession ID: MGI:7435289
Gene symbol: Slc12a2
Gene name: solute carrier family 12, member 2
Antibody: anti-NKCC1 (N-16) (sc-21545)
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-NKCC1 (T4) MGI:7435240 may have been used in this assay.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex
E16 +/+ E16.0 (a) TS24: cerebral cortex Present (b) involves: C57BL/6 Not Specified
P1 +/+ P1 TS27: cerebral cortex Present (b) involves: C57BL/6 Not Specified
P4 +/+ P4 TS28: cerebral cortex Weak (c) involves: C57BL/6 Not Specified
P7 +/+ P4 TS28: cerebral cortex Weak (c) involves: C57BL/6 Not Specified
E16. -/- E16.0 (a) TS24: cerebral cortex Strong (d) C57BL/6-Ecpasem1Plch Ecpasem1Plch/Ecpasem1PlchNot Specified
P1 -/- P1 TS27: cerebral cortex Strong (d) C57BL/6-Ecpasem1Plch Ecpasem1Plch/Ecpasem1PlchNot Specified
P4 -/- P4 TS28: cerebral cortex Present (e) C57BL/6-Ecpasem1Plch Ecpasem1Plch/Ecpasem1PlchNot Specified
P7 -/- P4 TS28: cerebral cortex Present (e) C57BL/6-Ecpasem1Plch Ecpasem1Plch/Ecpasem1PlchNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression at higher levels at embryonic and postnatal days before P4.
(c) Expression gradually decreased from P4.
(d) Signficantly higher expression compared with wildtype.
(e) Slower rate of decline compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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