Gene Expression Data
Assay Details
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Assay
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Reference:
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J:294897
Ankamreddy H, et al., CXCL12 is required for stirrup-shaped stapes formation during mammalian middle ear development. Dev Dyn. 2020 Sep;249(9):1117-1126
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7435231
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Probe:
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Pecam1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2E:
embryonic day 11.5
(more  )
Specimen
2E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Cxcl12tm1Spo/Cxcl12tm1Spo
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2P:
embryonic day 11.5
(more )
Specimen
2P:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: stapedial artery |
endothelial cell of artery |
Present |
Regionally restricted |
2P
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Expression was detected in the endothelial cells of the stapedial artery in the hole of the condensing stapedial mesenchyme.
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Specimen
2U:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Cxcl12tm1Spo/Cxcl12tm1Spo
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: stapedial artery |
endothelial cell of artery |
Present |
Regionally restricted |
2U
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Expression in the endothelial cells of the mutant stapedial artery in the hole of the condensing stapedial mesenchyme was similar to control.
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Specimen
2X:
embryonic day 12.5
(more )
Specimen
2X:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: stapedial artery |
endothelial cell of artery |
Present |
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2X
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Expression was detected in the endothelial cells of the stapedial artery in the hole of the condensing stapedial mesenchyme.
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Specimen
2Ab:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Cxcl12tm1Spo/Cxcl12tm1Spo
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3J:
embryonic day 18.5
(more )
Specimen
3J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3N:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Cxcl12tm1Spo/Cxcl12tm1Spo
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: stapes |
Absent |
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3N
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The stapedial artery was not observed in the mutant.
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Analysis Tools