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Gene Expression Data
Assay Details
Assay
Reference: J:294897 Ankamreddy H, et al., CXCL12 is required for stirrup-shaped stapes formation during mammalian middle ear development. Dev Dyn. 2020 Sep;249(9):1117-1126
Assay type: RNA in situ
MGI Accession ID: MGI:7435175
Gene symbol: Tfap2a
Gene name: transcription factor AP-2, alpha
Probe: Tfap2a cDNA
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 1B: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: 1st branchial arch mesenchyme derived from neural crest Present Regionally restricted 1B Expression was detected in the mesenchyme, dorsal to the malleus-incus condensation.
Specimen 1E: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: 1st branchial arch mesenchyme derived from neural crest Weak Regionally restricted 1E Expression was gradually decreased in the mesenchyme at E11.0.
Specimen 1H: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 1st branchial arch Absent 1H
Specimen 1L: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: 2nd branchial arch mesenchyme derived from neural crest Present Regionally restricted 1L In pharyngeal arch 2, the expression domain overlaps with stapedial condensation.
TS17: middle ear mesenchyme Present Regionally restricted 1L In pharyngeal arch 2, the expression domain overlaps with stapedial condensation.
Specimen 1O: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: 2nd branchial arch mesenchyme derived from neural crest Present Regionally restricted 1O In pharyngeal arch 2, the expression domain overlaps with stapedial condensation.
TS18: middle ear mesenchyme Present Regionally restricted 1O In pharyngeal arch 2, the expression domain overlaps with stapedial condensation.
Specimen 1R: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 2nd branchial arch mesenchyme derived from neural crest Present Regionally restricted 1R In pharyngeal arch 2, the expression domain overlaps with stapedial condensation.
TS19: middle ear mesenchyme Present Regionally restricted 1R In pharyngeal arch 2, the expression domain overlaps with stapedial condensation.
Specimen 1V: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: ossicle pre-cartilage condensation Weak Regionally restricted 1V Weak expression in the stapes region.
Specimen 1Y: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: middle ear Absent 1Y
Specimen 2B: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: 1st branchial arch mesenchyme derived from neural crest Present Regionally restricted 2B Expression was detected in the mesenchyme, dorsal to the malleus-incus condensation.
Specimen 2D: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 1st branchial arch Absent 2D
Specimen 2G: embryonic day 10.5; Cxcl12tm1Spo/Cxcl12tm1Spo (more )
Structure Level Pattern Image Note
TS17: 1st branchial arch mesenchyme derived from neural crest Present Regionally restricted 2G Expression in the mutant mesenchyme, dorsal to the malleus-incus condensation was similar to control.
Specimen 2I: embryonic day 11.5; Cxcl12tm1Spo/Cxcl12tm1Spo (more )
Structure Level Pattern Image Note
TS19: 1st branchial arch Absent 2I
Specimen 2M: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: 2nd branchial arch mesenchyme derived from neural crest Present Regionally restricted 2M In pharyngeal arch 2, the expression domain overlaps with stapedial condensation.
TS17: middle ear mesenchyme Present Regionally restricted 2M In pharyngeal arch 2, the expression domain overlaps with stapedial condensation.
Specimen 2O: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 2nd branchial arch mesenchyme derived from neural crest Present Regionally restricted 2O In pharyngeal arch 2, the expression domain overlaps with stapedial condensation.
TS19: middle ear mesenchyme Present Regionally restricted 2O In pharyngeal arch 2, the expression domain overlaps with stapedial condensation.
Specimen 2R: embryonic day 10.5; Cxcl12tm1Spo/Cxcl12tm1Spo (more )
Structure Level Pattern Image Note
TS17: 2nd branchial arch mesenchyme derived from neural crest Weak Regionally restricted 2R Stapedial expression was weaker at E10.5 in the mutant.
TS17: middle ear mesenchyme Weak Regionally restricted 2R Stapedial expression was weaker at E10.5 in the mutant.
Specimen 2T: embryonic day 11.5; Cxcl12tm1Spo/Cxcl12tm1Spo (more )
Structure Level Pattern Image Note
TS19: 2nd branchial arch mesenchyme derived from neural crest Present Regionally restricted 2T Mutant stapedial expression was similar to control at E11.5.
TS19: middle ear mesenchyme Present Regionally restricted 2T Mutant stapedial expression was similar to control at E11.5.
Specimen 4C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: middle ear mesenchyme Present Regionally restricted 4C Expression was detected in the prospective stapes region.
Specimen 4G: embryonic day 10.5; Bmp4tm1Blh/Bmp4tm4Blh, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: middle ear mesenchyme Present Regionally restricted 4G Expression was absent from the stapes region but was restricted to the lateral mesenchyme in the conditional mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory