Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:318994
Yang J, et al., Localization of Beclin1 in mouse developing tooth germs: possible implication of the interrelation between autophagy and apoptosis. J Mol Histol. 2013 Dec;44(6):619-27
|
Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7433070
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Gene symbol:
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Becn1
|
Gene name:
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beclin 1, autophagy related
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Antibody:
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anti-Beclin1
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Detection system:
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Secondary antibody coupled to rhodamine
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Assay notes:
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Cap stage of tooth development.
|
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Results
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Specimen
3a:
embryonic day 14.5
(more )
Note: Triple labeled: red - Becn1; blue - Map1lc3a (assay MGI:7433073); green - Casp3 (assay MGI:7433075).
Specimen
3a:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.5
|
Age Note:
|
`
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
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4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Triple labeled: red - Becn1; blue - Map1lc3a (assay MGI:7433073); green - Casp3 (assay MGI:7433075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: primary enamel knot |
Present |
Regionally restricted |
3f
,
3h'
,
3f'
,
3e'
,
3e
,
3h
,
3a
|
Expression is at the center.
|
TS22: stellate reticulum |
Present |
|
3a
,
3e
,
3h
,
3f'
,
3h'
,
3f
,
3e'
|
|
Specimen
3i:
embryonic day 14.5
(more )
Note: Triple labeled: red - Becn1; blue - Map1lc3a (assay MGI:7433073); green - Casp3 (assay MGI:7433075).
Specimen
3i:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Triple labeled: red - Becn1; blue - Map1lc3a (assay MGI:7433073); green - Casp3 (assay MGI:7433075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: palatal shelf epithelium |
Present |
Regionally restricted |
3l
,
3i
|
Expression is in the non-transitional epithelial island of the medial epithelial seam at the midline.
|
|