Probe:	Ucma cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Two probes were described MGI:7432816 and MGI:7432817; the one used in this assay was not detailed.

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present	Regionally restricted	4Ab , 4Aa	Expression was detected at the distal ends of vertebrae.

Structure	Level	Pattern	Image	Note
TS23: vertebra	Strong	Regionally restricted	4Ac , 4Ad	Expression was mostly on the surface of the vertebra.

Structure	Level	Pattern	Image	Note
TS23: vertebra	Strong	Regionally restricted	4Ae	Expression was mostly on the surface of the vertebra.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: tibia epiphysis	chondrocyte	Present	Regionally restricted	4Bd , 4Bc	Expression was detected in the epiphyses of tibiae in early differentiated chondrocytes in the epiphyseal surface zone.
TS23: long bone epiphyseal plate resting zone		Present	Regionally restricted	4Bd , 4Bc	Expression was observed in the distal and peripheral resting zone of epiphyseal cartilage of the developing limbs.
TS23: long bone epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Absent		4Bd , 4Bc

Structure	Cell Type	Level	Pattern	Image	Note
TS26: long bone epiphyseal plate proliferative zone	chondrocyte	Present	Regionally restricted	4Bh , 4Bg	Expression was detected in most chondrocytes of the upper and peripheral resting zone of the epiphysis, and to some extent in the laterally located proliferating chondrocytes. Signal was absent from the hypertrophic zone, the central part of the proliferating zone and the joint surface. Strongest expression resided in the periphery of the epiphyses.
TS26: long bone epiphyseal plate resting zone	chondrocyte	Present	Regionally restricted	4Bh , 4Bg	Expression was detected in most chondrocytes of the upper and peripheral resting zone of the epiphysis, and to some extent in the laterally located proliferating chondrocytes. Signal was absent from the hypertrophic zone, the central part of the proliferating zone and the joint surface. Strongest expression resided in the periphery of the epiphyses.
TS26: tibia epiphysis	chondrocyte	Present	Regionally restricted	4Bh , 4Bg	Expression was detected in most chondrocytes of the upper and peripheral resting zone of the epiphysis, and to some extent in the laterally located proliferating chondrocytes. Signal was absent from the hypertrophic zone, the central part of the proliferating zone and the joint surface. Strongest expression resided in the periphery of the epiphyses.
TS26: long bone epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Absent		4Bg , 4Bh

Structure	Cell Type	Level	Pattern	Image	Note
TS28: tibia epiphysis		Present	Regionally restricted	4Bk , 4Bl	Expression was restricted to the peripheral zone of epiphyseal cartilage and disappeared from the secondary ossification center of the epiphysis.
TS28: long bone epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Absent		4Bk , 4Bl

Structure	Cell Type	Level	Pattern	Image	Note
TS28: tibia epiphysis	chondrocyte	Present	Regionally restricted	4Bp , 4Bo	Expression was restricted to chondrocytes at the periphery of the growth plate.
TS28: long bone epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Absent		4Bo , 4Bp

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate proliferative zone	chondrocyte	Present	Single cells	4Bs , 4Bt	Only sporadic labeled cells were present in the epiphyseal growth zone.
TS28: tibia epiphysis	chondrocyte	Present	Single cells	4Bs , 4Bt	Only sporadic labeled cells were present in the epiphyseal growth zone.
TS28: long bone epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Absent		4Bs , 4Bt

Structure	Cell Type	Level	Pattern	Image	Note
TS27: humerus epiphysis	chondrocyte	Present	Regionally restricted	5a	Expression was detected in distal epiphyseal regions, predominantly in resting chondrocytes.
TS27: long bone epiphyseal plate resting zone	chondrocyte	Present	Regionally restricted	5a	Expression was detected in distal epiphyseal regions, predominantly in resting chondrocytes.
TS27: scapula		Present		5a

Structure	Cell Type	Level	Pattern	Image	Note
TS27: femur epiphysis	chondrocyte	Present	Regionally restricted	5d' , 5d	Expression was detected in distal epiphyseal regions, predominantly in resting chondrocytes.
TS27: long bone epiphyseal plate resting zone	chondrocyte	Present	Regionally restricted	5d' , 5d	Expression was detected in distal epiphyseal regions, predominantly in resting chondrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: long bone epiphyseal plate resting zone	chondrocyte	Present	Regionally restricted	5g	Expression was detected in distal epiphyseal regions, predominantly in resting chondrocytes.
TS27: tibia epiphysis	chondrocyte	Present	Regionally restricted	5g	Expression was detected in distal epiphyseal regions, predominantly in resting chondrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate resting zone	chondrocyte	Present	Regionally restricted	5j	Expression was detected in distal epiphyseal regions, predominantly in resting chondrocytes.
TS28: tibia epiphysis	chondrocyte	Present	Regionally restricted	5j	Expression was detected in distal epiphyseal regions, predominantly in resting chondrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: vertebra	chondrocyte	Present	Regionally restricted	S1a	Expression was detected in distal regions of vertebral cartilage rich in juvenile chondrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: sternum	chondrocyte	Present	Regionally restricted	S1d' , S1d	Expression was detected in distal regions of sternal cartilage rich in juvenile chondrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: rib	chondrocyte	Present	Regionally restricted	S1g	Expression was detected in distal regions of rib cartilage rich in juvenile chondrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: vertebra	chondrocyte	Present	Single cells		Backbone expression declined during development, and by postnatal day 60 only sporadic chondrocytes were stained.