Isl1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:75868 Lu QR, et al., Common developmental requirement for olig function indicates a motor neuron/oligodendrocyte connection. Cell. 2002 Apr 5;109(1):75-86
Assay type:	RNA in situ
MGI Accession ID:	MGI:7432589
Gene symbol:	Isl1
Gene name:	ISL1 transcription factor, LIM/homeodomain

Probe:	Isl1 cDNA
Probe preparation:	Antisense RNA

Results

Specimen 4A +/+ E9.5: embryonic day 9.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS15: neural tube lateral wall	somatomotor neuron	Present		4A-2
TS15: neural tube lateral wall	visceromotor neuron	Present		4A-2

Specimen 4A -/- E9.5: embryonic day 9.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	4A-5	A transient population of a few positive cells was noted in the lateral ventral neural tube.

Specimen 4A +/+ E11.5: embryonic day 11.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS19: neural tube lateral wall	somatomotor neuron	Present		4A-8
TS19: neural tube lateral wall	visceromotor neuron	Present		4A-8

Specimen 4A -/- E11.5: embryonic day 11.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Absent		4A-11

Specimen 4B +/+: embryonic day 18.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: hindbrain	somatomotor neuron	Present		4B +/+ Isl1
TS26: hindbrain	motor neuron	Present	Regionally restricted	4B +/+ Isl1	Expressed in preganglion motor neurons.

Specimen 4B -/-: embryonic day 18.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: hindbrain	somatomotor neuron	Absent		4B -/- Isl1
TS26: hindbrain	motor neuron	Present	Regionally restricted	4B -/- Isl1	Expression in the preganglion motor neurons was unaffected.

Specimen 4C +/+: embryonic day 18.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: hindbrain	motor neuron	Present	Regionally restricted	4C +/+	Expressed in the branchial motor neurons.

Specimen 4C -/-: embryonic day 18.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: hindbrain	motor neuron	Present	Regionally restricted	4C -/-	Expressed in the branchial motor neurons, similar as in the wild-type.

Specimen 7A: embryonic day 10.5; Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺, Olig1^tm1(cre)Rth/Olig1⁺ (more

)
Note: Conditional mutant. Double labeled: brown - Isl1; blue - Cre reporter.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: neural tube lateral wall	spinal cord motor neuron	Present		7A 10.5 LacZ Isl1	Colocalization with Olig1-Cre linage expression was seen.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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