Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75868
Lu QR, et al., Common developmental requirement for olig function indicates a motor neuron/oligodendrocyte connection. Cell. 2002 Apr 5;109(1):75-86
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7432589
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Probe:
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Isl1 cDNA
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Probe preparation:
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Antisense RNA
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Results
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Specimen
4A +/+ E9.5:
embryonic day 9.5
(more  )
Specimen
4A +/+ E9.5:
(close  )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
4A -/- E9.5:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube lateral wall |
Present |
Regionally restricted |
4A-5
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A transient population of a few positive cells was noted in the lateral ventral neural tube.
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Specimen
4A +/+ E11.5:
embryonic day 11.5
(more )
Specimen
4A +/+ E11.5:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
4A -/- E11.5:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
4B +/+:
embryonic day 18.5
(more )
Specimen
4B +/+:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: hindbrain |
somatomotor neuron |
Present |
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4B Isl1 +/+
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| TS26: hindbrain |
motor neuron |
Present |
Regionally restricted |
4B Isl1 +/+
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Expressed in preganglion motor neurons.
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Specimen
4B -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: hindbrain |
somatomotor neuron |
Absent |
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4B Isl1 -/-
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| TS26: hindbrain |
motor neuron |
Present |
Regionally restricted |
4B Isl1 -/-
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Expression in the preganglion motor neurons was unaffected.
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Specimen
4C +/+:
embryonic day 18.5
(more )
Specimen
4C +/+:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: hindbrain |
motor neuron |
Present |
Regionally restricted |
4C +/+
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Expressed in the branchial motor neurons.
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Specimen
4C -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: hindbrain |
motor neuron |
Present |
Regionally restricted |
4C -/-
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Expressed in the branchial motor neurons, similar as in the wild-type.
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Specimen
7A:
embryonic day 10.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Olig1tm1(cre)Rth/Olig1 +
(more )
Note: Conditional mutant. Double labeled: brown - Isl1; blue - Cre reporter.
Specimen
7A:
(close )
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Genetic Background:
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involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6J
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Mutant Allele(s):
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Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Olig1tm1(cre)Rth/Olig1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Double labeled: brown - Isl1; blue - Cre reporter.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
spinal cord motor neuron |
Present |
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7A 10.5 LacZ Isl1
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Colocalization with Olig1-Cre linage expression was seen.
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Analysis Tools