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Gene Expression Data
Assay Details
Assay
Reference: J:309500 Jokoji G, et al., CDC5L promotes early chondrocyte differentiation and proliferation by modulating pre-mRNA splicing of SOX9, COL2A1, and WEE1. J Biol Chem. 2021 Aug;297(2):100994
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7432402
Gene symbol: Pcna
Gene name: proliferating cell nuclear antigen
Antibody: anti-PCNA (ab29)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-CDC5L (HPA011361) MGI:7432395 may have been used in this assay.
Results
Specimen 2B Pcna: embryonic day 17.5 (more )
Note: humeral head
Structure Cell Type Level Pattern Image Note
TS25: humerus chondrocyte Present Regionally restricted 2B Pcna , 2B merge Co-expressed with Cdc5l in ~75% of chondrocytes.
Specimen 2C Pcna: embryonic day 17.5 (more )
Note: columnar chondrocytes
Structure Cell Type Level Pattern Image Note
TS25: humerus columnar chondrocyte Present Regionally restricted 2C Pcna , 2C merge Co-expressed with Cdc5l in ~75% of chondrocytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory