Gene Expression Data
Assay Details
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Assay
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Reference:
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J:309500
Jokoji G, et al., CDC5L promotes early chondrocyte differentiation and proliferation by modulating pre-mRNA splicing of SOX9, COL2A1, and WEE1. J Biol Chem. 2021 Aug;297(2):100994
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7432402
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Gene symbol:
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Pcna
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Gene name:
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proliferating cell nuclear antigen
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Antibody:
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anti-PCNA (ab29)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. Anti-CDC5L (HPA011361) MGI:7432395 may have been used in this assay.
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Results
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Specimen
2B Pcna:
embryonic day 17.5
(more  )
Note: humeral head
Specimen
2B Pcna:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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humeral head
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: humerus |
chondrocyte |
Present |
Regionally restricted |
2B Pcna
,
2B merge
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Co-expressed with Cdc5l in ~75% of chondrocytes.
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Specimen
2C Pcna:
embryonic day 17.5
(more )
Note: columnar chondrocytes
Specimen
2C Pcna:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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columnar chondrocytes
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: humerus |
columnar chondrocyte |
Present |
Regionally restricted |
2C Pcna
,
2C merge
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Co-expressed with Cdc5l in ~75% of chondrocytes.
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Analysis Tools
