Calb1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:332658 Keeley PW, et al., Neurog2 regulates Isl1 to modulate horizontal cell number. Development. 2023 Jan 1;150(1):dev201315
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7431226
Gene symbol:	Calb1
Gene name:	calbindin 1

Antibody:	anti-Calbindin D-28K (Ab-1) (PC253L)
Detection system:	Secondary antibody
Assay notes:	Control mice were littermates lacking either floxed or Chx10-cre alleles.

Results

3B CTRL ©

Specimen 3B CTRL: postnatal week 6-8 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		3B CTRL

3B CKO ©

Specimen 3B CKO: postnatal week 6-8; Neurog2^{tm5(Neurog2)Fgu}/Neurog2^{tm5(Neurog2)Fgu}, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		3B CKO	Significantly fewer horizontal cells compared to control.

3E CTRL ©

Specimen 3E CTRL: postnatal week 6-8 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		3E CTRL

3E CKO ©

Specimen 3E CKO: postnatal week 6-8; Neurog2^{tm5(Neurog2)Fgu}/Neurog2^{tm5(Neurog2)Fgu}, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present	Regionally restricted	3E CKO	Characteristic expression in stratified horizontal cell somata and processes.

5B CTRL ©

Specimen 5B CTRL: postnatal day 5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		5B CTRL

5B DCKO ©

Specimen 5B DCKO: postnatal day 5; Isl1^tm2Sev/Isl1^tm2Sev, Neurog2^{tm5(Neurog2)Fgu}/Neurog2^{tm5(Neurog2)Fgu}, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		5B DCKO	Significant increase in horizontal cell number.

5E CTRL ©

Specimen 5E CTRL: postnatal day 5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		5E CTRL

5E DCKO ©

Specimen 5E DCKO: postnatal day 5; Isl1^tm2Sev/Isl1^tm2Sev, Neurog2^{tm5(Neurog2)Fgu}/Neurog2^{tm5(Neurog2)Fgu}, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present	Regionally restricted	5E DCKO	Stratification and differentation of horizontal cells appeared unaltered.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/14/2024 MGI 6.23