Slc6a19 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:332480 Treleaven T, et al., Stage-Specific L-Proline Uptake by Amino Acid Transporter Slc6a19/B(0)AT1 Is Required for Optimal Preimplantation Embryo Development in Mice. Cells. 2022 Dec 21;12(1)
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7431178
Gene symbol:	Slc6a19
Gene name:	solute carrier family 6 (neurotransmitter transporter), member 19

Antibody:	anti-B⁰AT1
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

1_1-cell ©

Specimen 1 1-cell: embryonic day 0.5 (more

)
Note: Also stained for F-actin using phalloidin (green) to outline the periphery of each blastomere.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		1_1-cell

1_2-cell ©

Specimen 1 2-cell: embryonic day 1.0 (more

)
Note: Also stained for F-actin using phalloidin (green) to outline the periphery of each blastomere.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		1_2-cell

1_4-cell ©

Specimen 1 4-cell: embryonic day 2.0 (more

)
Note: Also stained for F-actin using phalloidin (green) to outline the periphery of each blastomere.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present		1_4-cell

1_8-cell ©

Specimen 1 8-cell: embryonic day 2.5 (more

)
Note: Also stained for F-actin using phalloidin (green) to outline the periphery of each blastomere.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		1_8-cell

1_Morula ©

Specimen 1 Morula: embryonic day 3.0 (more

)
Note: Also stained for F-actin using phalloidin (green) to outline the periphery of each blastomere.

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		1_Morula

1_Blastocyst ©

Specimen 1 Blastocyst: embryonic day 3.5 (more

)
Note: Also stained for F-actin using phalloidin (green) to outline the periphery of each blastomere.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		1_Blastocyst

2A B⁰AT1 1-cell ©

2A Overlay 1-cell ©

Specimen 2A 1-cell: embryonic day 0.5 (more

)
Note: Double labeled: red - Slc6a19; green - Ace2 (assay MGI:7431170)

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Non-Uniform	2A B⁰AT1 1-cell , 2A Overlay 1-cell

2A B⁰AT1 2-cell ©

2A Overlay 2-cell ©

Specimen 2A 2-cell: embryonic day 1.0 (more

)
Note: Double labeled: red - Slc6a19; green - Ace2 (assay MGI:7431170)

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		2A B⁰AT1 2-cell , 2A Overlay 2-cell

2A B⁰AT1 4-cell ©

2A Overlay 4-cell ©

Specimen 2A 4-cell: embryonic day 2.0 (more

)
Note: Double labeled: red - Slc6a19; green - Ace2 (assay MGI:7431170)

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present		2A Overlay 4-cell , 2A B⁰AT1 4-cell

2A B⁰AT1 8-cell ©

2A Overlay 8-cell ©

Specimen 2A 8-cell: embryonic day 2.5 (more

)
Note: Double labeled: red - Slc6a19; green - Ace2 (assay MGI:7431170)

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		2A Overlay 8-cell , 2A B⁰AT1 8-cell

2A B⁰AT1 Morula ©

2A Overlay Morula ©

Specimen 2A Morula: embryonic day 3.0 (more

)
Note: Double labeled: red - Slc6a19; green - Ace2 (assay MGI:7431170)

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		2A Overlay Morula , 2A B⁰AT1 Morula

2A B⁰AT1 Blastocyst ©

2A Overlay Blastocyst ©

Specimen 2A Blastocyst: embryonic day 3.5 (more

)
Note: Double labeled: red - Slc6a19; green - Ace2 (assay MGI:7431170)

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		2A B⁰AT1 Blastocyst , 2A Overlay Blastocyst

2B B⁰AT1 1-cell ©

2B Overlay 1-cell ©

Specimen 2B 1-cell: embryonic day 0.5 (more

)
Note: Double labeled: red - Slc6a19; green - Cltrn (assay MGI:7431173)

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		2B B⁰AT1 1-cell , 2B Overlay 1-cell

2B B⁰AT1 2-cell ©

2B Overlay 2-cell ©

Specimen 2B 2-cell: embryonic day 1.0 (more

)
Note: Double labeled: red - Slc6a19; green - Cltrn (assay MGI:7431173)

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		2B B⁰AT1 2-cell , 2B Overlay 2-cell

2B B⁰AT1 4-cell ©

2B Overlay 4-cell ©

Specimen 2B 4-cell: embryonic day 2.0 (more

)
Note: Double labeled: red - Slc6a19; green - Cltrn (assay MGI:7431173)

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present		2B Overlay 4-cell , 2B B⁰AT1 4-cell

Specimen 2B 8-cell: embryonic day 2.5 (more

)
Note: Double labeled: red - Slc6a19; green - Cltrn (assay MGI:7431173)

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		2B B⁰AT1 8-cell , 2B Overlay 8-cell

Specimen 2B Morula: embryonic day 3.0 (more

)
Note: Double labeled: red - Slc6a19; green - Cltrn (assay MGI:7431173)

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		2B B⁰AT1 Morula , 2B Overlay Morula

Specimen 2B Blastocyst: embryonic day 3.5 (more

)
Note: Double labeled: red - Slc6a19; green - Cltrn (assay MGI:7431173)

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		2B Overlay Blastocyst , 2B B⁰AT1 Blastocyst

3Ai COC ©

Specimen 3Ai COC: postnatal week 4-8 (more

)

Structure	Level	Pattern	Image	Note
TS28: cumulus oophorus	Present		3Ai COC

Specimen 3Ai Blastocyst: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		3Ai Blastocyst

3Aii COC ©

Specimen 3Aii COC: postnatal week 4-8; Slc6a19^tm1Dgen/Slc6a19^tm1Dgen (more

)

Structure	Level	Pattern	Image	Note
TS28: cumulus oophorus	Absent		3Aii COC

Specimen 3Aii Blastocyst: embryonic day 3.5; Slc6a19^tm1Dgen/Slc6a19^tm1Dgen (more

)

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Absent		3Aii Blastocyst

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