Probe:	beta domain probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: cardiac muscle tissue	Present		1A/B
TS13: heart	Present	Regionally restricted	1A/B	Expression is in the myocardium and is absent in the endocardium.
TS13: dorsal mesocardium	Present	Regionally restricted	1A/B	Expression is in the splanchnic lining of the dorsal mesocardium.

Structure	Level	Pattern	Image	Note
TS14: primitive ventricle	Present	Non-Uniform	1C	The presumptive right ventricle expresses at a higher level than the left ventricle.
TS14: outflow tract	Present	Regionally restricted	1C	Expression is in the myocardium.
TS14: primitive ventricle cardiac muscle	Present		1C
TS14: common atrial chamber	Weak		1C

Structure	Level	Pattern	Image	Note
TS16: primitive ventricle cardiac muscle	Present	Non-Uniform	1D	The presumptive right ventricle expresses at a higher level than the left ventricle.
TS16: heart atrium	Present	Regionally restricted	1D	Expression is in the roof of the atrium where a thickening marks the site of formation of the primary septum.
TS16: atrioventricular endocardial cushion	Present		1D

Structure	Level	Pattern	Image	Note
TS17: atrioventricular endocardial cushion	Strong		1E
TS17: interatrial septum	Present		1E
TS17: primitive ventricle cardiac muscle	Present	Non-Uniform	1E	The presumptive right ventricle expresses at a higher level than the left ventricle.
TS17: atrium cardiac muscle	Absent		1E

Structure	Level	Pattern	Image	Note
TS17: outflow tract endocardial cushion	Present		1F
TS17: outflow tract	Present	Regionally restricted	1F	Expression is in the developing truncal ridges.
TS17: primitive ventricle cardiac muscle	Present	Non-Uniform	1F	The presumptive right ventricle expresses at a higher level than the left ventricle.
TS17: atrium cardiac muscle	Absent		1F

Structure	Level	Pattern	Image	Note
TS19: atrioventricular endocardial cushion	Strong		1G
TS19: right ventricle cardiac muscle	Present		1G
TS19: left ventricle cardiac muscle	Weak		1G
TS19: atrium cardiac muscle	Absent		1G

Structure	Level	Pattern	Image	Note
TS19: right ventricle cardiac muscle	Strong		1H
TS19: left ventricle cardiac muscle	Present		1H
TS19: atrium cardiac muscle	Absent		1H

Structure	Level	Pattern	Image	Note
TS19: outflow tract endocardial cushion	Present		1I
TS19: left ventricle cardiac muscle	Present		1I
TS19: right ventricle cardiac muscle	Present		1I
TS19: interatrial septum	Present	Regionally restricted	1I	Expression is strong in the leading edge and is absent in the main body of the septum.
TS19: heart	Present	Regionally restricted	1I	Expression is in the developing valves guarding the entry of the superior caval vein into the right atrium.
TS19: atrium cardiac muscle	Absent		1I

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Strong		2A
TS20: heart valve primordium	Present	Regionally restricted	2A	Expression is in the leaflets of the venous valves.
TS20: left ventricle cardiac muscle	Weak		2A
TS20: right ventricle cardiac muscle	Weak		2A

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Strong		2B
TS20: interventricular septum	Present	Regionally restricted	2B	The core of the ventricular septum has downregulated expression, whereas the crest and outer layers of the septum are positive.
TS20: heart left ventricle	Weak		2B
TS20: heart right ventricle	Weak		2B

Structure	Level	Pattern	Image	Note
TS21: endocardial cushion	Strong		2C/D
TS21: mitral valve	Strong		2C/D
TS21: tricuspid valve	Strong		2C/D
TS21: right atrium valve	Present		2C/D
TS21: interventricular septum	Present	Non-Uniform	2C/D	Expression is strong at the crest , whereas the core of the septum has lower levels than the outer layers.
TS21: left ventricle cardiac muscle	Weak		2C/D
TS21: right ventricle cardiac muscle	Weak		2C/D
TS21: septum primum	Absent		2C/D

Structure	Level	Pattern	Image	Note
TS21: atrioventricular endocardial cushion	Strong		2E
TS21: semilunar valve	Strong		2E
TS21: aortico-pulmonary spiral septum	Absent		2E

Structure	Level	Pattern	Image	Note
TS27: atrioventricular valve	Present		2F
TS27: mitral valve	Present		2F
TS27: tricuspid valve	Present		2F
TS27: heart left ventricle	Absent		2F
TS27: heart right ventricle	Absent		2F

Structure	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	Present	Regionally restricted	3A/B	Expression is in the developing trabeculations and is weak in the myocardial wall.

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Strong		3C/D	Expression is in the developing trabeculations and in the myocardial wall.
TS19: trabecular myocardium	Strong		3C/D	Expression is in the developing trabeculations and in the myocardial wall.

Structure	Level	Pattern	Image	Note
TS21: trabecular myocardium	Present		3E/F
TS21: myocardium layer	Weak		3E/F	Expression is in the trabeculations and is downregulated in both the compact and spongy layers of the myocardial wall.

Structure	Level	Pattern	Image	Note
TS21: septum secundum	Absent