Antibody:	anti-Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb #4631
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Uniform	3I , 3M
TS11: embryo mesoderm	Present	Regionally restricted	3M , 3I	Expression was less uniform and was position dependent. In some areas, most of the mesoderm cells were positive.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Uniform	3J , 3N
TS11: embryo mesoderm	Present	Regionally restricted	3N , 3J	Expression was less uniform and was position dependent. In distal areas, entire mesoderm layer was negative.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Uniform	3K , 3O
TS11: embryo mesoderm	Present	Regionally restricted	3K , 3O	Expression was less uniform and was position dependent. In some areas, most of the mesoderm cells were positive.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Weak	Regionally restricted	3L , 3P	In some areas, the embryonic ectoderm was negative.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Weak	Regionally restricted	3Q , 3R	Only a small region on distal end was positive. Most of the ectoderm cells were negative.