Toe1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:332468 Wang H, et al., Knockdown of Toe1 causes developmental arrest during the morula-to-blastocyst transition in mice. Theriogenology. 2022 Dec;194:154-161
Assay type:	RT-PCR
MGI Accession ID:	MGI:7429018
Gene symbol:	Toe1
Gene name:	target of EGR1, member 1 (nuclear)

Probe:	Toe1-pA, Toe1-pB
Assay notes:	Quantitative real time PCR was used to obtain these results. Transcript levels were normalized against Gapdh. Expression levels for zygotes were used for calibration.

Results

Image: 1A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
zy	E0.5 (a)	TS1: 1-cell stage conceptus	Weak	Not Specified; total RNA	involves: C57BL/6 * DBA/2		Not Specified
2C	E1.0 (a)	TS2: 2-cell stage conceptus	Weak	Not Specified; total RNA	involves: C57BL/6 * DBA/2		Not Specified
4C	E2.0 (a)	TS3: 4-cell stage conceptus	Present	Not Specified; total RNA	involves: C57BL/6 * DBA/2		Not Specified
8C	E2.5 (a)	TS3: 8-cell stage conceptus	Present	Not Specified; total RNA	involves: C57BL/6 * DBA/2		Not Specified
Mo	E3.0 (a)	TS3: morula-stage conceptus	Present	Not Specified; total RNA	involves: C57BL/6 * DBA/2		Not Specified
BL	E3.5 (a)	TS4: blastocyst-stage conceptus	Weak	Not Specified; total RNA	involves: C57BL/6 * DBA/2		Not Specified

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Levels of mRNA gradually increase from the two-cell stage, peak at the eight-cell stage, and then reduce.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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