Gene Expression Data
Assay Details
Assay
|
Reference:
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J:331872
Johnson GL, et al., En1 and Lmx1b do not recapitulate embryonic dorsal-ventral limb patterning functions during mouse digit tip regeneration. Cell Rep. 2022 Nov 22;41(8):111701
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7427810
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Gene symbol:
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Lmx1b
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Gene name:
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LIM homeobox transcription factor 1 beta
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Probe:
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Lmx1b cDNA7
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Probe preparation:
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Antisense RNA
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Assay notes:
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Hybridization chain reaction RNA fluorescent in situ hybridization (HCR RNA-FISH).
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Results
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Specimen
1B:
embryonic day 16.5
(more )
Note: Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
Specimen
1B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: digit mesenchyme |
Present |
Regionally restricted |
1B
,
1B'
,
1B''
|
Expression is dorsal and is absent in the distal or ventral mesenchyme.
|
Specimen
1C:
embryonic day 16.5
(more )
Note: Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
Specimen
1C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: digit mesenchyme |
Present |
Regionally restricted |
1C''
,
1C
,
1C'
|
Expression is dorsal and is absent in the distal or ventral mesenchyme.
|
Specimen
1D:
embryonic day 16.5
(more )
Note: Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
Specimen
1D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: digit mesenchyme |
Present |
Regionally restricted |
1D
,
1D''
,
1D'
|
Expression is dorsal and is absent in the distal or ventral mesenchyme.
|
Specimen
1E:
postnatal day 4.5
(more )
Note: Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
Specimen
1E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 4.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: digit mesenchyme |
Present |
Regionally restricted |
1E'
,
1E
,
1E''
|
Expression is dorsal and is absent at the distal tip of the digit or ventrally.
|
Specimen
1F:
postnatal day 4.5
(more )
Note: Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
Specimen
1F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 4.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: digit mesenchyme |
Present |
Regionally restricted |
1F
,
1F'
,
1F''
|
Expression is dorsal and is absent at the distal tip of the digit or ventrally.
|
Specimen
1G:
postnatal day 4.5
(more )
Note: Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
Specimen
1G:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 4.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: digit mesenchyme |
Present |
Regionally restricted |
1G'
,
1G''
,
1G
|
Expression is dorsal and is absent at the distal tip of the digit or ventrally.
|
Specimen
1H:
postnatal week 6
(more )
Note: Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
Specimen
1H:
(close )
Genetic Background:
|
FVB/NJ
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: digit mesenchyme |
Present |
Regionally restricted |
1H
,
1H''
,
1H'
|
Expression is in the dorsal mesenchyme and has extended distally to the tip and is absent in the ventral mesenchyme.
|
Specimen
1I:
postnatal week 6
(more )
Note: Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
Specimen
1I:
(close )
Genetic Background:
|
FVB/NJ
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: digit mesenchyme |
Present |
Regionally restricted |
1I'
,
1I''
,
1I
|
Expression is in the dorsal mesenchyme and has extended distally to the tip and is absent in the ventral mesenchyme.
|
Specimen
1J:
postnatal week 6
(more )
Note: Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
Specimen
1J:
(close )
Genetic Background:
|
FVB/NJ
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: digit mesenchyme |
Present |
Regionally restricted |
1J
,
1J'
,
1J''
|
Expression is in the dorsal mesenchyme and has extended distally to the tip and is absent in the ventral mesenchyme.
|
Specimen
S1B:
embryonic day 10.5
(more )
Note: Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
Specimen
S1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Lmx1b; green - En1 (assay MGI:7427808).
|
|