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Gene Expression Data
Assay Details
Assay
Reference: J:331872 Johnson GL, et al., En1 and Lmx1b do not recapitulate embryonic dorsal-ventral limb patterning functions during mouse digit tip regeneration. Cell Rep. 2022 Nov 22;41(8):111701
Assay type: RNA in situ
MGI Accession ID: MGI:7427808
Gene symbol: En1
Gene name: engrailed 1
Probe: En1 cDNA7
Probe preparation: Antisense RNA
Assay notes: Hybridization chain reaction RNA fluorescent in situ hybridization (HCR RNA-FISH).
Results
Specimen 1B: embryonic day 16.5 (more )
Note: Double labeled: green - En1; magenta - Lmx1b (assay MGI:7427810).
Structure Level Pattern Image Note
TS24: digit skin Present Regionally restricted 1B , 1B'' , 1B' Expression is dorsal to the dorsal-ventral boundary at the distal tip of the digit and is absent in the rest of the dorsal epithelium.
Specimen 1C: embryonic day 16.5 (more )
Note: Double labeled: green - En1; magenta - Lmx1b (assay MGI:7427810).
Structure Level Pattern Image Note
TS24: digit skin Present Regionally restricted 1C , 1C'' , 1C' Expression is dorsal to the dorsal-ventral boundary at the distal tip of the digit and is absent in the rest of the dorsal epithelium.
Specimen 1D: embryonic day 16.5 (more )
Note: Double labeled: green - En1; magenta - Lmx1b (assay MGI:7427810).
Structure Level Pattern Image Note
TS24: digit skin Present Regionally restricted 1D , 1D' , 1D'' Expression is in the ventral limb bud epithelium.
Specimen 1E: postnatal day 4.5 (more )
Note: Double labeled: green - En1; magenta - Lmx1b (assay MGI:7427810).
Structure Level Pattern Image Note
TS28: digit skin Present Regionally restricted 1E' , 1E , 1E'' Expression is ventral and at the distal tip and has expanded further dorsally.
Specimen 1F: postnatal day 4.5 (more )
Note: Double labeled: green - En1; magenta - Lmx1b (assay MGI:7427810).
Structure Level Pattern Image Note
TS28: digit skin Present Regionally restricted 1F'' , 1F' , 1F Expression is ventral and at the distal tip and has expanded further dorsally.
Specimen 1G: postnatal day 4.5 (more )
Note: Double labeled: green - En1; magenta - Lmx1b (assay MGI:7427810).
Structure Level Pattern Image Note
TS28: digit skin Present Regionally restricted 1G' , 1G , 1G'' Expression is ventral and at the distal tip and has expanded further dorsally.
Specimen 1H: postnatal week 6 (more )
Note: Double labeled: green - En1; magenta - Lmx1b (assay MGI:7427810).
Structure Level Pattern Image Note
TS28: digit skin Present 1H , 1H'' , 1H' Expression is throughout the entire digit tip epithelium.
Specimen 1I: postnatal week 6 (more )
Note: Double labeled: green - En1; magenta - Lmx1b (assay MGI:7427810).
Structure Level Pattern Image Note
TS28: digit skin Present 1I' , 1I'' , 1I Expression is throughout the entire digit tip epithelium.
Specimen 1J: postnatal week 6 (more )
Note: Double labeled: green - En1; magenta - Lmx1b (assay MGI:7427810).
Structure Level Pattern Image Note
TS28: digit skin Present 1J , 1J' , 1J'' Expression is throughout the entire digit tip epithelium.
Specimen S1B: embryonic day 10.5 (more )
Note: Double labeled: green - En1; magenta - Lmx1b (assay MGI:7427810).
Structure Level Pattern Image Note
TS17: limb ectoderm Present Regionally restricted S1B' , S1B'' , S1B Expression is in the ventral limb bud epithelium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory