Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:253585
Siletti K, et al., Daple coordinates organ-wide and cell-intrinsic polarity to pattern inner-ear hair bundles. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11170-E11179
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7427543
|
|
Gene symbol:
|
Pard3
|
|
Gene name:
|
par-3 family cell polarity regulator
|
|
Antibody:
|
anti-PAR-3
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
|
Assay notes:
|
Fixation was with TCA.
|
|
|
Results
|
Specimen
8E Apex:
embryonic day 16.5
(more  )
Note: Double labeled: green - Pard3; magenta - Daple (Ccdc88c assay MGI:7427530).
Specimen
8E Apex:
(close  )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
Formaldehyde
|
|
Note:
|
Double labeled: green - Pard3; magenta - Daple (Ccdc88c assay MGI:7427530).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: cochlea |
cochlea auditory hair cell |
Present |
Regionally restricted |
8E Apex Merged
,
8E Apex Par-3
|
Expression enriched at neural edges of several immature outer hair cells at apex of cochlea.
|
| TS24: cochlea |
supporting cell of cochlea |
Present |
Regionally restricted |
8E Apex Merged
,
8E Apex Par-3
|
Junctional expression in supporting cells with no obvious planar orientation.
|
Specimen
8E Base:
embryonic day 16.5
(more )
Note: Double labeled: green - Pard3; magenta - Daple (Ccdc88c assay MGI:7427530).
Specimen
8E Base:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
Formaldehyde
|
|
Note:
|
Double labeled: green - Pard3; magenta - Daple (Ccdc88c assay MGI:7427530).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: cochlea |
cochlea auditory hair cell |
Present |
Regionally restricted |
8E Base Merged
,
8E Base Par-3
|
Near base of cochlea, expression distributed abneurally in more mature hair cells.
|
| TS24: cochlea |
supporting cell of cochlea |
Present |
Regionally restricted |
8E Base Par-3
,
8E Base Merged
|
Junctional expression in supporting cells with no obvious planar orientation.
|
|
Analysis Tools