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Gene Expression Data
Assay Details
Assay
Reference: J:253585 Siletti K, et al., Daple coordinates organ-wide and cell-intrinsic polarity to pattern inner-ear hair bundles. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11170-E11179
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7427532
Gene symbol: Gnai3
Gene name: G protein subunit alpha i3
Antibody: Anti-G-alpha-i3 (G4040)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: The authors used three anti-Gnai3 antibodies but did not detail which was used to obtain these data; anti-Gai-3 (sc-262) (sc-137416) MGI:7427459 or anti-GNAI3 MGI:7427460 may have been used instead to generate these data.
Results
Specimen 2A Daple+/-: postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Double labeled: green - Gnai3; magenta - LGN (Gpsm2 assay MGI:7427535).
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 2A Daple+/- Expression colocalized with LGN (Gpsm2) at apical surfaces of hair cells.
Specimen 2A Daple-/-: postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Double labeled: green - Gnai3; magenta - LGN (Gpsm2 assay MGI:7427535).
Structure Cell Type Level Pattern Image Note
TS27: cochlea cochlea auditory hair cell Present Regionally restricted 2A Daple-/- Expression colocalized with LGN (Gpsm2) at apical surfaces of hair cells.
Specimen 2B Daple+/-: postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Double labeled: green - Gnai3; magenta - aPKC (Prkcz assay MGI:7427536).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 2B Daple+/- Expression domain complementary to that of aPKC (Prkcz).
Specimen 2B Daple-/-: postnatal day 1; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Double labeled: green - Gnai3; magenta - aPKC (Prkcz assay MGI:7427536).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 2B Daple-/- Expression domain complementary to that of aPKC (Prkcz).
Specimen 5E Daple+/-: embryonic day 17.5; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Double labeled: green - Gnai3; magenta - Pcnt (assay MGI:7427538).
Structure Cell Type Level Pattern Image Note
TS25: cochlea cochlea auditory hair cell Present Regionally restricted 5E Daple+/- Expression at middle cochlear turn. Expression domains misoriented in some hair cells.
Specimen 5E Daple-/-: embryonic day 17.5; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Double labeled: green - Gnai3; magenta - Pcnt (assay MGI:7427538).
Structure Cell Type Level Pattern Image Note
TS25: cochlea cochlea auditory hair cell Present Regionally restricted 5E Daple-/- Expression domains comparably oriented compared to that of control.
Specimen 5F Daple+/-: embryonic day 17.5; Ccdc88ctm1(KOMP)Mbp/Ccdc88c+ (more )
Note: Double labeled: green - Gnai3; magenta - Pcnt (assay MGI:7427538).
Structure Cell Type Level Pattern Image Note
TS25: cochlea cochlea auditory hair cell Present Regionally restricted 5F Daple+/- Expression in hair cells at cochlear bases.
Specimen 5F Daple-/-: embryonic day 17.5; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Note: Double labeled: green - Gnai3; magenta - Pcnt (assay MGI:7427538).
Structure Cell Type Level Pattern Image Note
TS25: cochlea cochlea auditory hair cell Present Regionally restricted 5F Daple-/- Distribution of expression similar to that of heterozygous hair cells.
Specimen 8A (top): postnatal day 2 (more )
Note: Triple labeled: green - Gnai3; magenta - Daple (Ccdc88c assay MGI:7427530); blue - actin.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8A (top) Expression localizes more apical that Daple (Ccdc88c).
Specimen 8A (bottom): postnatal day 2 (more )
Note: Triple labeled: green - Gnai3; magenta - Daple (Ccdc88c assay MGI:7427530); blue - actin.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8A (bottom) Expression localizes more apical that Daple (Ccdc88c).
Specimen 8D Apex: embryonic day 16.5 (more )
Note: Triple labeled: green - Gnai3; magenta - Daple (Ccdc88c assay MGI:7427530); blue - tubulin.
Structure Cell Type Level Pattern Image Note
TS24: cochlea cochlea auditory hair cell Present Regionally restricted 8D Apex Galphai , 8D Apex Merged Expression in many immature hair cells near cochlear apex. In some cells, expression is not assymmetrically distributed; in other cells, expression at neural edge.
Specimen 8D Middle: embryonic day 16.5 (more )
Note: Triple labeled: green - Gnai3; magenta - Daple (Ccdc88c assay MGI:7427530); blue - tubulin.
Structure Cell Type Level Pattern Image Note
TS24: cochlea cochlea auditory hair cell Present Regionally restricted 8D Middle Galphai , 8D Middle Merged In middle of cochlea, expression confined to abneural halves of some hair cells.
Specimen 8D Base: embryonic day 16.5 (more )
Note: Triple labeled: green - Gnai3; magenta - Daple (Ccdc88c assay MGI:7427530); blue - tubulin.
Structure Level Pattern Image Note
TS24: cochlea Present Regionally restricted 8D Base Merged , 8D Base Galphai Near base of cochlea, expression domains expanded in surface area.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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