Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:330271
Tekko T, et al., Assessing Myf5 and Lbx1 contribution to carapace development by reproducing their turtle-specific signatures in mouse embryos. Dev Dyn. 2022 Oct;251(10):1698-1710
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7423786
|
|
Gene symbol:
|
Myf6
|
|
Gene name:
|
myogenic factor 6
|
|
Probe:
|
Myf6 probe2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
4A/A':
embryonic day 9.0
(more  )
Specimen
4A/A':
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: myotome |
Present |
Regionally restricted |
4A
,
4A'
|
Expression followed both rostral to caudal and epaxial to hypaxial sequences, starting in the epaxial myotome of rostral somites.
|
Specimen
4C/C':
(close )
|
Genetic Background:
|
involves: FVB/NJ
|
|
Mutant Allele(s):
|
Myf5em2Mllo/Myf5em2Mllo
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: myotome |
Present |
Regionally restricted |
4C'
,
4C
|
Expression was prematurely activated in the hypaxial border of trunk somites. Epaxial expression in the more rostral somites was reduced when compared with control embryos.
|
Specimen
4B:
embryonic day 10.5
(more )
Specimen
4B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4D:
(close )
|
Genetic Background:
|
involves: FVB/NJ
|
|
Mutant Allele(s):
|
Myf5em2Mllo/Myf5em2Mllo
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: myotome |
Present |
Regionally restricted |
4D
|
Expression in trunk somites was mostly downregulated in the medial and hypaxial regions.
|
Specimen
4E/E':
embryonic day 10.5
(more )
Specimen
4E/E':
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Gelatin
|
Specimen
4F/F':
(close )
|
Genetic Background:
|
involves: FVB/NJ
|
|
Mutant Allele(s):
|
Myf5em2Mllo/Myf5em2Mllo
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Gelatin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk myotome |
Present |
Regionally restricted |
4F
,
4F'
|
Expression in trunk somites was mostly downregulated in the medial and hypaxial regions. Apparent alteration in the morphology of the dermomyotomal hypaxial lip was seen.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: FVB/NJ
|
|
Mutant Allele(s):
|
Myf5em3Mllo/Myf5em3Mllo
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: myotome |
Present |
|
|
Expression was prematurely activated in the hypaxial border of trunk somites. Epaxial expression in the more rostral somites was reduced when compared with control embryos.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: FVB/NJ
|
|
Mutant Allele(s):
|
Myf5em3Mllo/Myf5em3Mllo
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: myotome |
Present |
|
|
Expression in trunk somites was mostly downregulated in the medial and hypaxial regions.
|
|
Analysis Tools