Antibody:	anti-Tbx2 (D-3; sc-514291)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present	Widespread	1c E12.5 SOX2	Expression is seen in the prosensory epithelium in the floor of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct epithelium	Present	Widespread	1c E13.5 SOX2	Expression is seen in the prosensory epithelium in the floor of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present	Non-Uniform	1c E14.5 SOX2	Expression is seen in the prosensory epithelium. Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct epithelium	Present	Non-Uniform	1c E15.5 SOX2	Expression is seen in the prosensory epithelium. Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	1c E18.5 SOX2	Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.
TS26: greater epithelial ridge	Present		1c E18.5 SOX2	Expression is weaker than in the organ of Corti.
TS26: lesser epithelial ridge	Present		1c E18.5 SOX2	Expression is weaker than in the organ of Corti.

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present	Widespread	1c E12.5 CDH2	Expression is seen in the prosensory epithelium in the floor of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct epithelium	Present	Widespread	1c E13.5 CDH2	Expression is seen in the prosensory epithelium in the floor of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present	Non-Uniform	1c E14.5 CDH2	Expression is seen in the prosensory epithelium. Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct epithelium	Present	Non-Uniform	1c E15.5 CDH2	Expression is seen in the prosensory epithelium. Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	1c E18.5 CDH2	Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.
TS26: greater epithelial ridge	Present		1c E18.5 CDH2	Expression is weaker than in the organ of Corti.
TS26: lesser epithelial ridge	Present		1c E18.5 CDH2	Expression is weaker than in the organ of Corti.

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present	Widespread	1c E12.5 CDH1	Expression is seen in the prosensory epithelium in the floor of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct epithelium	Present	Widespread	1c E13.5 CDH1	Expression is seen in the prosensory epithelium in the floor of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present	Non-Uniform	1c E14.5 CDH1	Expression is seen in the prosensory epithelium. Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct epithelium	Present	Non-Uniform	1c E15.5 CDH1	Expression is seen in the prosensory epithelium. Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	1c E18.5 CDH1	Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.
TS26: greater epithelial ridge	Present		1c E18.5 CDH1	Expression is weaker than in the organ of Corti.
TS26: lesser epithelial ridge	Present		1c E18.5 CDH1	Expression is weaker than in the organ of Corti.

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present	Widespread	1c E12.5 MYO7A	Expression is seen in the prosensory epithelium in the floor of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct epithelium	Present	Widespread	1c E13.5 MYO7A	Expression is seen in the prosensory epithelium in the floor of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present	Non-Uniform	1c E14.5 MYO7A	Expression is seen in the prosensory epithelium. Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct epithelium	Present	Non-Uniform	1c E15.5 MYO7A	Expression is seen in the prosensory epithelium. Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	1c E18.5 MYO7A	Expression is strongly upregulated in the inner (medial) region of the developing organ of Corti; it is excluded from the outer (lateral) region.
TS26: greater epithelial ridge	Present		1c E18.5 MYO7A	Expression is weaker than in the organ of Corti.
TS26: lesser epithelial ridge	Present		1c E18.5 MYO7A	Expression is weaker than in the organ of Corti.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: organ of Corti	cochlear inner hair cell	Present		1d E18.5 MYO7A
TS26: organ of Corti	cochlear outer hair cell	Absent		1d E18.5 MYO7A

Structure	Cell Type	Level	Pattern	Image	Note
TS26: organ of Corti	cochlear inner hair cell	Present		1d E18.5 SLC17A8
TS26: organ of Corti	cochlear outer hair cell	Absent		1d E18.5 SLC17A8

Structure	Cell Type	Level	Pattern	Image	Note
TS26: organ of Corti	cochlear inner hair cell	Present		1d E18.5 BCL11B	Expression is confined to inner hair and supporting cells.
TS26: organ of Corti	cochlear outer hair cell	Absent		1d E18.5 BCL11B

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	1d E18.5 PROX1	Expression is confined to inner hair and supporting cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	cochlear inner hair cell	Present		1d P21 MYO7A
TS28: organ of Corti	cochlear outer hair cell	Absent		1d P21 MYO7A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	border cell of cochlea	Present		1d P21 CALB2
TS28: organ of Corti	cochlear inner hair cell	Present		1d P21 CALB2
TS28: organ of Corti	inner phalangeal cell	Present		1d P21 CALB2

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	border cell of cochlea	Present		1d P21 SLC26A5
TS28: organ of Corti	cochlear inner hair cell	Present		1d P21 SLC26A5
TS28: organ of Corti	inner phalangeal cell	Present		1d P21 SLC26A5
TS28: organ of Corti	cochlear outer hair cell	Absent		1d P21 SLC26A5

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti	border cell of cochlea	Present		1d P21 KCNQ4
TS28: organ of Corti	cochlear inner hair cell	Present		1d P21 KCNQ4
TS28: organ of Corti	inner phalangeal cell	Present		1d P21 KCNQ4
TS28: organ of Corti	cochlear outer hair cell	Absent		1d P21 KCNQ4