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Gene Expression Data
Assay Details
Assay
Reference: J:154510 Gebauer JM, et al., Mouse AMACO, a kidney and skin basement membrane associated molecule that mediates RGD-dependent cell attachment. Matrix Biol. 2009 Oct;28(8):456-62
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7414228
Gene symbol: Vwa2
Gene name: von Willebrand factor A domain containing 2
Antibody: anti-AMACO-P3
Detection system: Secondary antibody coupled to colloidal gold
Assay notes: Fix - 1.5% glutaraldehyde, 1.5% paraformaldehyde. Embedded in Spurr's epoxy.
Results
Specimen 2A,B: postnatal newborn (more )
Note: Fix - 1.5% glutaraldehyde, 1.5% paraformaldehyde. Embedded in Spurr's epoxy.
Structure Level Pattern Image Note
TS27: basement membrane Present Regionally restricted 2A , 2B Ultrastructural localization of label was detected on the mesenchymal side of tubular basement membranes.
TS27: renal tubule Present Regionally restricted 2A , 2B Ultrastructural localization of label was detected on the mesenchymal side of tubular basement membranes.
Specimen 4A: postnatal adult (more )
Note: Fix - 1.5% glutaraldehyde, 1.5% paraformaldehyde. Embedded in Spurr's epoxy.
Structure Level Pattern Image Note
TS28: epidermal-dermal junction Present Regionally restricted 4A Ultrastructural localization of label was detected at the sublamina densa within 150 nm of the basal lamina.
Specimen 4B: postnatal adult (more )
Note: Fix - 1.5% glutaraldehyde, 1.5% paraformaldehyde. Embedded in Spurr's epoxy.
Structure Level Pattern Image Note
TS28: epidermal-dermal junction Present Regionally restricted 4B Signal was observed close to the entry points of collagen VII-containing anchoring fibrils where they insert into the basement membrane.
Specimen 5B: postnatal adult (more )
Note: Fix - 1.5% glutaraldehyde, 1.5% paraformaldehyde. Embedded in Spurr's epoxy.
Structure Level Pattern Image Note
TS28: epidermal-dermal junction Present 5B
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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