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Gene Expression Data
Assay Details
Assay
Reference: J:161576 Zhong SC, et al., Dynamic expression and heterogeneous intracellular location of En-1 during late mouse embryonic development. Cells Tissues Organs. 2010;191(4):289-300
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7414213
Gene symbol: En1
Gene name: engrailed 1
Antibody: anti-En1 (C-19)
Detection system: Secondary antibody coupled to rhodamine
Assay notes: Fixative was 4% paraformaldehyde, 20% picric acid.
Results
Specimen 6B: postnatal day 7 (more )
Note: Double labeled: red - En1; green - Th (assay MGI:7414176).
Structure Cell Type Level Pattern Image Note
TS28: midbrain dopaminergic neuron Present 6C , 6C' , 6B Expression is nuclear.

Specimen 6E: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: vertebra Present Regionally restricted 6E Expression is in the nonnuclear subcellular regions in the rostral edges of the developing vertebrae.

Specimen 6F: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: vertebra Present Regionally restricted 6F' , 6F Expression is in the nonnuclear subcellular regions in the rostral edges of the developing vertebrae.

Specimen S1B: embryonic day 14.5 (more )
Note: Double labeled: red - En1; green - Th (assay MGI:7414176).
Structure Cell Type Level Pattern Image Note
TS22: midbrain dopaminergic neuron Present S1B , S1C Expression is nuclear.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory