Gene Expression Data
Assay Details
Assay
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Reference:
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J:161576
Zhong SC, et al., Dynamic expression and heterogeneous intracellular location of En-1 during late mouse embryonic development. Cells Tissues Organs. 2010;191(4):289-300
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7414213
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Gene symbol:
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En1
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Gene name:
|
engrailed 1
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Antibody:
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anti-En1 (C-19)
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Detection system:
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Secondary antibody coupled to rhodamine
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Assay notes:
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Fixative was 4% paraformaldehyde, 20% picric acid.
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Results
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Specimen
6B:
postnatal day 7
(more )
Note: Double labeled: red - En1; green - Th (assay MGI:7414176).
Specimen
6B:
(close )
Genetic Background:
|
BALB/c
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Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - En1; green - Th (assay MGI:7414176).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: midbrain |
dopaminergic neuron |
Present |
|
6C
,
6C'
,
6B
|
Expression is nuclear.
|
Specimen
6E:
embryonic day 14.5
(more )
Specimen
6E:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: vertebra |
Present |
Regionally restricted |
6E
|
Expression is in the nonnuclear subcellular regions in the rostral edges of the developing vertebrae.
|
Specimen
6F:
embryonic day 14.5
(more )
Specimen
6F:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: vertebra |
Present |
Regionally restricted |
6F'
,
6F
|
Expression is in the nonnuclear subcellular regions in the rostral edges of the developing vertebrae.
|
Specimen
S1B:
embryonic day 14.5
(more )
Note: Double labeled: red - En1; green - Th (assay MGI:7414176).
Specimen
S1B:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - En1; green - Th (assay MGI:7414176).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
dopaminergic neuron |
Present |
|
S1B
,
S1C
|
Expression is nuclear.
|
|