Gene Expression Data
Assay Details
Assay
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Reference:
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J:320760
Gomez-Fernandez C, et al., Calcium signaling in mouse oocyte maturation: the roles of STIM1, ORAI1 and SOCE. Mol Hum Reprod. 2012 Apr;18(4):194-203
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7414081
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Gene symbol:
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Orai1
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Gene name:
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ORAI calcium release-activated calcium modulator 1
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Antibody:
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Anti-ORAI1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
5B GV equatorial section:
postnatal week 8-12
(more )
Note: germinal vesicle (GV).
Specimen
5B GV equatorial section:
(close )
Genetic Background:
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(C57BL/6 x DBA/2)F1
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Age:
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postnatal week 8-12
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Sex:
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Female
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Type:
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optical section
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Fixation:
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Paraformaldehyde
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Note:
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germinal vesicle (GV).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: primary oocyte |
Present |
Regionally restricted |
5B GV equatorial section
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Expression of this plasma membrane-resident protein was restricted to the periphery of the oocyte, where it was found uniformly distributed.
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Specimen
5B GV total projection:
postnatal week 8-12
(more )
Note: germinal vesicle (GV). Images were obtained after combining all the confocal imaging sections for each oocyte (total projection of 35 z-sections).
Specimen
5B GV total projection:
(close )
Genetic Background:
|
(C57BL/6 x DBA/2)F1
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Age:
|
postnatal week 8-12
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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germinal vesicle (GV). Images were obtained after combining all the confocal imaging sections for each oocyte (total projection of 35 z-sections).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: primary oocyte |
Present |
Regionally restricted |
5B GV total projection
|
Expression of this plasma membrane-resident protein was restricted to the periphery of the oocyte, where it was found uniformly distributed.
|
Specimen
5B MII equatorial section:
postnatal week 8-12
(more )
Note: fully mature oocyte (MII oocytes).
Specimen
5B MII equatorial section:
(close )
Genetic Background:
|
(C57BL/6 x DBA/2)F1
|
Age:
|
postnatal week 8-12
|
Sex:
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Female
|
Type:
|
optical section
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Fixation:
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Paraformaldehyde
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Note:
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fully mature oocyte (MII oocytes).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: secondary oocyte |
Present |
Clusters |
5B MII equatorial section
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Signal reached a marked level of clustering in MII oocytes.
|
Specimen
5B MII total projection:
postnatal week 8-12
(more )
Note: fully mature oocyte (MII oocytes). Images were obtained after combining all the confocal imaging sections for each oocyte (total projection of 35 z-sections).
Specimen
5B MII total projection:
(close )
Genetic Background:
|
(C57BL/6 x DBA/2)F1
|
Age:
|
postnatal week 8-12
|
Sex:
|
Female
|
Type:
|
whole mount
|
Fixation:
|
Paraformaldehyde
|
Note:
|
fully mature oocyte (MII oocytes). Images were obtained after combining all the confocal imaging sections for each oocyte (total projection of 35 z-sections).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: secondary oocyte |
Present |
Clusters |
5B MII total projection
|
Signal reached a marked level of clustering in MII oocytes.
|
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