Dicer1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:203415 Flemr M, et al., A retrotransposon-driven dicer isoform directs endogenous small interfering RNA production in mouse oocytes. Cell. 2013 Nov 7;155(4):807-16
Assay type:	RT-PCR
MGI Accession ID:	MGI:7412993
Gene symbol:	Dicer1
Gene name:	dicer 1, ribonuclease type III

Probe:	Dicer-pA, Dicer-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Hprt.

Results

Image: 2B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
PO	P adult	TS28: oocyte	Present	Not Specified; total RNA	involves: C57BL/6J * CBA/CaJ		Female
GO	P adult	TS28: oocyte	Strong (a)	Not Specified; total RNA	involves: C57BL/6J * CBA/CaJ		Female
FGO	P adult	TS28: oocyte	Strong (a)	Not Specified; total RNA	involves: C57BL/6J * CBA/CaJ		Female
1C	E0.5 (b)	TS1: 1-cell stage embryo	Strong	Not Specified; total RNA	involves: C57BL/6J * CBA/CaJ		Not Specified
2C	E1.0 (b)	TS2: 2-cell stage embryo	Trace (c)	Not Specified; total RNA	involves: C57BL/6J * CBA/CaJ		Not Specified
8C	E2.5 (b)	TS3: 8-cell stage embryo	Trace	Not Specified; total RNA	involves: C57BL/6J * CBA/CaJ		Not Specified
BL	E3.5 (b)	TS4: blastocyst	Trace	Not Specified; total RNA	involves: C57BL/6J * CBA/CaJ		Not Specified

Notes:
(a) Expression level increased during oocyte growth.
(b) Age assigned by curator based on morphological criteria supplied by authors.
(c) Expresssion level rapidly declined after fertilization.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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