Hbb-b1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:190446 Brown FC, et al., ENU mutagenesis identifies the first mouse mutants reproducing human beta-thalassemia at the genomic level. Blood Cells Mol Dis. 2013 Feb;50(2):86-92
Assay type:	Northern blot
MGI Accession ID:	MGI:7412931
Gene symbol:	Hbb-b1
Gene name:	hemoglobin, beta adult major chain

Probe:	beta-major probe2
Probe preparation:	labelled with P32

Results

Image: 3C

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	1400 bp	778 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
RBC14 WT	E16.5	TS24: liver	Absent (a)	Strong	10 µg; total RNA	involves: FVB/N		Not Specified
RBC14 WT	E16.5	TS24: liver	Absent (a)	Strong	10 µg; total RNA	involves: FVB/N		Not Specified
RBC14 +/-	E16.5	TS24: liver	Trace	Present (b)	10 µg; total RNA	involves: FVB/N	Hbb-b1^MommeD7/Hbb-b1⁺	Not Specified
RBC14 +/-	E16.5	TS24: liver	Trace	Present (b)	10 µg; total RNA	involves: FVB/N	Hbb-b1^MommeD7/Hbb-b1⁺	Not Specified
RBC14 -/-	E16.5	TS24: liver	Trace (c)	Weak (d)	10 µg; total RNA	involves: FVB/N	Hbb-b1^MommeD7/Hbb-b1^MommeD7	Not Specified
RBC14 -/-	E16.5	TS24: liver	Trace (c)	Weak (d)	10 µg; total RNA	involves: FVB/N	Hbb-b1^MommeD7/Hbb-b1^MommeD7	Not Specified

Notes:
(a) Longer transcript absent in wild-type.
(b) Expression level 68% that of wild-type littermates.
(c) Mutants display a longer RNA transcript compared to normal. Expression much lower compared to reduction of normal transcript, suggsting that the longer transcript is less stable.
(d) Expression level 35% that of wild-type littermates.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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