Hbb-b1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:190446 Brown FC, et al., ENU mutagenesis identifies the first mouse mutants reproducing human beta-thalassemia at the genomic level. Blood Cells Mol Dis. 2013 Feb;50(2):86-92
Assay type:	Northern blot
MGI Accession ID:	MGI:7412931
Gene symbol:	Hbb-b1
Gene name:	hemoglobin, beta adult major chain

Probe:	beta-major probe2
Probe preparation:	labelled with P32

Results

Image: 3C

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	1400 bp	778 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
RBC14 WT	E16.5	TS24: liver	Absent (a)	Strong	10 µg; total RNA	involves: FVB/N		Not Specified
RBC14 WT	E16.5	TS24: liver	Absent (a)	Strong	10 µg; total RNA	involves: FVB/N		Not Specified
RBC14 +/-	E16.5	TS24: liver	Trace	Present (b)	10 µg; total RNA	involves: FVB/N	Hbb-b1^MommeD7/Hbb-b1⁺	Not Specified
RBC14 +/-	E16.5	TS24: liver	Trace	Present (b)	10 µg; total RNA	involves: FVB/N	Hbb-b1^MommeD7/Hbb-b1⁺	Not Specified
RBC14 -/-	E16.5	TS24: liver	Trace (c)	Weak (d)	10 µg; total RNA	involves: FVB/N	Hbb-b1^MommeD7/Hbb-b1^MommeD7	Not Specified
RBC14 -/-	E16.5	TS24: liver	Trace (c)	Weak (d)	10 µg; total RNA	involves: FVB/N	Hbb-b1^MommeD7/Hbb-b1^MommeD7	Not Specified

Notes:
(a) Longer transcript absent in wild-type.
(b) Expression level 68% that of wild-type littermates.
(c) Mutants display a longer RNA transcript compared to normal. Expression much lower compared to reduction of normal transcript, suggsting that the longer transcript is less stable.
(d) Expression level 35% that of wild-type littermates.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24