Antibody:	anti-S100A1 (C0318-1)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS22: urothelium of bladder	Absent		3_S100A1 E14.5 , 3_NP63/S100A1 E14.5

Structure	Level	Pattern	Image	Note
TS22: urothelium of bladder	Absent		3_UPK1B/S100A1 E14.5

Structure	Cell Type	Level	Pattern	Image	Note
TS23: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_S100A1 E15.5 , 3_NP63/S100A1 E15.5	Epithelial expression commenced in some luminal cells at E15.5.
TS23: basal cell layer of bladder urothelium		Absent		3_NP63/S100A1 E15.5 , 3_S100A1 E15.5
TS23: intermediate cell layer of bladder urothelium		Absent		3_NP63/S100A1 E15.5 , 3_S100A1 E15.5

Structure	Cell Type	Level	Pattern	Image	Note
TS23: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_UPK1B/S100A1 E15.5	Epithelial expression commenced in some luminal cells at E15.5. Expression overlapped with that of Upk1b in luminal cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Widespread	3_S100A1 E16.5 , 3_NP63/S100A1 E16.5	Expression in entire luminal cell layer from E16.5 onwards
TS24: basal cell layer of bladder urothelium		Absent		3_NP63/S100A1 E16.5 , 3_S100A1 E16.5
TS24: intermediate cell layer of bladder urothelium		Absent		3_NP63/S100A1 E16.5 , 3_S100A1 E16.5

Structure	Cell Type	Level	Pattern	Image	Note
TS24: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Widespread	3_UPK1B/S100A1 E16.5	Expression in entire luminal cell layer from E16.5 onwards. Expression overlapped with that of Upk1b in luminal cells. Expression overlapped with that of Upk1b in luminal cells.
TS24: basal cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 E16.5
TS24: intermediate cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 E16.5

Structure	Cell Type	Level	Pattern	Image	Note
TS25: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_NP63/S100A1 E17.5 , 3_S100A1 E17.5	Expression restricted to cytoplasm of superficial (S-) layer cells.
TS25: basal cell layer of bladder urothelium		Absent		3_NP63/S100A1 E17.5 , 3_S100A1 E17.5
TS25: intermediate cell layer of bladder urothelium		Absent		3_NP63/S100A1 E17.5 , 3_S100A1 E17.5

Structure	Cell Type	Level	Pattern	Image	Note
TS25: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_UPK1B/S100A1 E17.5	Expression restricted to cytoplasm of superficial (S-) layer cells. Expression overlapped with that of Upk1b in luminal cells. Expression overlapped with that of Upk1b in luminal cells.
TS25: basal cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 E17.5
TS25: intermediate cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 E17.5

Structure	Cell Type	Level	Pattern	Image	Note
TS26: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_NP63/S100A1 E18.5 , 3_S100A1 E18.5	Expression restricted to cytoplasm of superficial (S-) layer cells.
TS26: basal cell layer of bladder urothelium		Absent		3_S100A1 E18.5 , 3_NP63/S100A1 E18.5
TS26: intermediate cell layer of bladder urothelium		Absent		3_S100A1 E18.5 , 3_NP63/S100A1 E18.5

Structure	Cell Type	Level	Pattern	Image	Note
TS26: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_UPK1B/S100A1 E18.5	Expression restricted to cytoplasm of superficial (S-) layer cells. Expression overlapped with that of Upk1b in luminal cells.
TS26: basal cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 E18.5
TS26: intermediate cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 E18.5

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_S100A1 P7 , 3_NP63/S100A1 P7	Expression restricted to cytoplasm of superficial (S-) layer cells.
TS28: basal cell layer of bladder urothelium		Absent		3_NP63/S100A1 P7 , 3_S100A1 P7
TS28: intermediate cell layer of bladder urothelium		Absent		3_NP63/S100A1 P7 , 3_S100A1 P7

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_UPK1B/S100A1 P7	Expression restricted to cytoplasm of superficial (S-) layer cells. Expression overlapped with that of Upk1b in luminal cells. Expression overlapped with that of Upk1b in luminal cells.
TS28: basal cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 P7
TS28: intermediate cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 P7

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_S100A1 P14 , 3_NP63/S100A1 P14	Expression restricted to cytoplasm of superficial (S-) layer cells.
TS28: basal cell layer of bladder urothelium		Absent		3_S100A1 P14 , 3_NP63/S100A1 P14
TS28: intermediate cell layer of bladder urothelium		Absent		3_S100A1 P14 , 3_NP63/S100A1 P14

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_UPK1B/S100A1 P14	Expression restricted to cytoplasm of superficial (S-) layer cells. Expression overlapped with that of Upk1b in luminal cells.
TS28: basal cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 P14
TS28: intermediate cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 P14

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_S100A1 P40 , 3_NP63/S100A1 P40	Expression restricted to cytoplasm of superficial (S-) layer cells.
TS28: basal cell layer of bladder urothelium		Absent		3_NP63/S100A1 P40 , 3_S100A1 P40
TS28: intermediate cell layer of bladder urothelium		Absent		3_NP63/S100A1 P40 , 3_S100A1 P40

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	3_UPK1B/S100A1 P40	Expression restricted to cytoplasm of superficial (S-) layer cells. Expression overlapped with that of Upk1b in luminal cells.
TS28: basal cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 P40
TS28: intermediate cell layer of bladder urothelium		Absent		3_UPK1B/S100A1 P40

Structure	Level	Pattern	Image	Note
TS22: urothelium of bladder	Absent		S2_NP63/S100A1 E14.5 , S2_S100A1 E14.5

Structure	Level	Pattern	Image	Note
TS22: urothelium of bladder	Absent		S2_UPK1B/S100A1 E14.5

Structure	Cell Type	Level	Pattern	Image	Note
TS23: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_S100A1 E15.5 , S2_NP63/S100A1 E15.5	Epithelial expression commenced in some luminal cells at E15.5.
TS23: basal cell layer of bladder urothelium		Absent		S2_S100A1 E15.5 , S2_NP63/S100A1 E15.5
TS23: intermediate cell layer of bladder urothelium		Absent		S2_S100A1 E15.5 , S2_NP63/S100A1 E15.5

Structure	Cell Type	Level	Pattern	Image	Note
TS23: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_UPK1B/S100A1 E15.5	Epithelial expression commenced in some luminal cells at E15.5. Expression overlapped with that of Upk1b in luminal cells.
TS23: basal cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 E15.5
TS23: intermediate cell layer of ureter		Absent		S2_UPK1B/S100A1 E15.5

Structure	Cell Type	Level	Pattern	Image	Note
TS24: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Widespread	S2_NP63/S100A1 E16.5 , S2_S100A1 E16.5	Expression in entire luminal cell layer from E16.5 onwards
TS24: basal cell layer of bladder urothelium		Absent		S2_NP63/S100A1 E16.5 , S2_S100A1 E16.5
TS24: intermediate cell layer of bladder urothelium		Absent		S2_NP63/S100A1 E16.5 , S2_S100A1 E16.5

Structure	Cell Type	Level	Pattern	Image	Note
TS24: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Widespread	S2_UPK1B/S100A1 E16.5	Expression in entire luminal cell layer from E16.5 onwards. Expression overlapped with that of Upk1b in luminal cells.
TS24: basal cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 E16.5
TS24: intermediate cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 E16.5

Structure	Cell Type	Level	Pattern	Image	Note
TS25: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_NP63/S100A1 E17.5 , S2_S100A1 E17.5	Expression restricted to cytoplasm of superficial (S-) layer cells.
TS25: basal cell layer of bladder urothelium		Absent		S2_S100A1 E17.5 , S2_NP63/S100A1 E17.5
TS25: intermediate cell layer of bladder urothelium		Absent		S2_S100A1 E17.5 , S2_NP63/S100A1 E17.5

Structure	Cell Type	Level	Pattern	Image	Note
TS25: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_UPK1B/S100A1 E17.5	Expression restricted to cytoplasm of superficial (S-) layer cells. Expression overlapped with that of Upk1b in luminal cells.
TS25: basal cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 E17.5
TS25: intermediate cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 E17.5

Structure	Cell Type	Level	Pattern	Image	Note
TS26: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_NP63/S100A1 E18.5 , S2_S100A1 E18.5	Expression restricted to cytoplasm of superficial (S-) layer cells.
TS26: basal cell layer of bladder urothelium		Absent		S2_NP63/S100A1 E18.5 , S2_S100A1 E18.5
TS26: intermediate cell layer of bladder urothelium		Absent		S2_NP63/S100A1 E18.5 , S2_S100A1 E18.5

Structure	Cell Type	Level	Pattern	Image	Note
TS26: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_UPK1B/S100A1 E18.5	Expression restricted to cytoplasm of superficial (S-) layer cells. Expression overlapped with that of Upk1b in luminal cells.
TS26: basal cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 E18.5
TS26: intermediate cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 E18.5

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_NP63/S100A1 P7 , S2_S100A1 P7	Expression restricted to cytoplasm of superficial (S-) layer cells.
TS28: basal cell layer of bladder urothelium		Absent		S2_NP63/S100A1 P7 , S2_S100A1 P7
TS28: intermediate cell layer of bladder urothelium		Absent		S2_NP63/S100A1 P7 , S2_S100A1 P7

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_UPK1B/S100A1 P7	Expression restricted to cytoplasm of superficial (S-) layer cells. Expression overlapped with that of Upk1b in luminal cells.
TS28: basal cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 P7
TS28: intermediate cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 P7

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_NP63/S100A1 P14 , S2_S100A1 P14	Expression restricted to cytoplasm of superficial (S-) layer cells.
TS28: basal cell layer of bladder urothelium		Absent		S2_S100A1 P14 , S2_NP63/S100A1 P14
TS28: intermediate cell layer of bladder urothelium		Absent		S2_S100A1 P14 , S2_NP63/S100A1 P14

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_UPK1B/S100A1 P14	Expression restricted to cytoplasm of superficial (S-) layer cells. Expression overlapped with that of Upk1b in luminal cells.
TS28: basal cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 P14
TS28: intermediate cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 P14

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_NP63/S100A1 P40 , S2_S100A1 P40	Expression restricted to cytoplasm of superficial (S-) layer cells.
TS28: basal cell layer of bladder urothelium		Absent		S2_S100A1 P40 , S2_NP63/S100A1 P40
TS28: intermediate cell layer of bladder urothelium		Absent		S2_S100A1 P40 , S2_NP63/S100A1 P40

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Present	Regionally restricted	S2_UPK1B/S100A1 P40	Expression restricted to cytoplasm of superficial (S-) layer cells. Expression overlapped with that of Upk1b in luminal cells.
TS28: basal cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 P40
TS28: intermediate cell layer of bladder urothelium		Absent		S2_UPK1B/S100A1 P40

Structure	Level	Pattern	Image	Note
TS22: urothelium of ureter	Absent		4_S100A1/NP63 E14.5 , 4_S100A1 E14.5

Structure	Level	Pattern	Image	Note
TS22: urothelium of ureter	Absent		4_S100A1/UPK1B E14.5

Structure	Level	Pattern	Image	Note
TS22: urothelium of bladder	Absent		4_S100A/KRT5 E14.5

Structure	Level	Pattern	Image	Note
TS23: urothelium of ureter	Absent		4_S100A1/NP63 E15.5 , 4_S100A1 E15.5

Structure	Level	Pattern	Image	Note
TS23: urothelium of ureter	Absent		4_S100A1/UPK1B E15.5

Structure	Level	Pattern	Image	Note
TS23: urothelium of ureter	Absent		4_S100A/KRT5 E15.5

Structure	Level	Pattern	Image	Note
TS24: urothelium of ureter	Absent		4_S100A1 E16.5 , 4_S100A1/NP63 E16.5

Structure	Level	Pattern	Image	Note
TS24: urothelium of ureter	Absent		4_S100A1/UPK1B E16.5

Structure	Level	Pattern	Image	Note
TS24: urothelium of ureter	Absent		4_S100A/KRT5 E16.5

Structure	Cell Type	Level	Pattern	Image	Note
TS25: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A1 E17.5 , 4_S100A1/NP63 E17.5	At E17.5, expression in some luminal cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A1/UPK1B E17.5	At E17.5, expression in some luminal cells. Expression overlapped with that of Upk1b in luminal cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: superficial cell layer of ureter	bladder urothelial cell	Present	Regionally restricted	4_S100A/KRT5 E17.5

Structure	Cell Type	Level	Pattern	Image	Note
TS26: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A1 E18.5 , 4_S100A1/NP63 E18.5	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells.
TS26: basal cell layer of ureter		Absent		4_S100A1/NP63 E18.5 , 4_S100A1 E18.5
TS26: intermediate cell layer of ureter		Absent		4_S100A1/NP63 E18.5 , 4_S100A1 E18.5

Structure	Cell Type	Level	Pattern	Image	Note
TS26: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A1/UPK1B E18.5	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells. Expression overlapped with that of Upk1b in luminal cells.
TS26: basal cell layer of ureter		Absent		4_S100A1/UPK1B E18.5
TS26: intermediate cell layer of ureter		Absent		4_S100A1/UPK1B E18.5

Structure	Cell Type	Level	Pattern	Image	Note
TS26: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A/KRT5 E18.5	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells.
TS26: basal cell layer of ureter		Absent		4_S100A/KRT5 E18.5
TS26: intermediate cell layer of ureter		Absent		4_S100A/KRT5 E18.5

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A1/NP63 P7 , 4_S100A1 P7	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells.
TS28: basal cell layer of ureter		Absent		4_S100A1/NP63 P7 , 4_S100A1 P7
TS28: intermediate cell layer of ureter		Absent		4_S100A1/NP63 P7 , 4_S100A1 P7

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A1/UPK1B P7	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells. Expression overlapped with that of Upk1b in luminal cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A/KRT5 P7	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells.
TS28: basal cell layer of ureter		Absent		4_S100A/KRT5 P7
TS28: intermediate cell layer of ureter		Absent		4_S100A/KRT5 P7

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A1 P14 , 4_S100A1/NP63 P14	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells.
TS28: basal cell layer of ureter		Absent		4_S100A1 P14 , 4_S100A1/NP63 P14
TS28: intermediate cell layer of ureter		Absent		4_S100A1 P14 , 4_S100A1/NP63 P14

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A1/UPK1B P14	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells. Expression overlapped with that of Upk1b in luminal cells.
TS28: basal cell layer of ureter		Absent		4_S100A1/UPK1B P14
TS28: intermediate cell layer of ureter		Absent		4_S100A1/UPK1B P14

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A/KRT5 P14	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells.
TS28: basal cell layer of ureter		Absent		4_S100A/KRT5 P14
TS28: intermediate cell layer of ureter		Absent		4_S100A/KRT5 P14

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A1 P40 , 4_S100A1/NP63 P40	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells.
TS28: basal cell layer of ureter		Absent		4_S100A1/NP63 P40 , 4_S100A1 P40
TS28: intermediate cell layer of ureter		Absent		4_S100A1/NP63 P40 , 4_S100A1 P40

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A1/UPK1B P40	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells. Expression overlapped with that of Upk1b in luminal cells.
TS28: basal cell layer of ureter		Absent		4_S100A1/UPK1B P40
TS28: intermediate cell layer of ureter		Absent		4_S100A1/UPK1B P40

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	4_S100A/KRT5 P40	Expression restricted to nucleus and cytoplasm of all superficial (S-) luminal cells.
TS28: basal cell layer of ureter		Absent		4_S100A/KRT5 P40
TS28: intermediate cell layer of ureter		Absent		4_S100A/KRT5 P40