Probe:	S100a1 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: urothelium of bladder	Weak	Regionally restricted	1_S100a1 E14.5	Weak expression first detected at luminal side of epithelium, predominantly 2-layered at this stage.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		1_S100a1 E15.5	From E15.5 to P40, strong expression confined to luminal cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		1_S100a1 E16.5	From E15.5 to P40, strong expression confined to luminal cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		1_S100a1 E17.5	From E15.5 to P40, strong expression confined to luminal cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		1_S100a1 E18.5	From E15.5 to P40, strong expression confined to luminal cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		1_S100a1 P7	From E15.5 to P40, strong expression confined to luminal cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		1_S100a1 P14	From E15.5 to P40, strong expression confined to luminal cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		1_S100a1 P40	From E15.5 to P40, strong expression confined to luminal cell layer.

Structure	Level	Pattern	Image	Note
TS22: urothelium of bladder	Weak	Regionally restricted	S1_S100a1 E14.5	Weak expression first detected at luminal side of epithelium, predominantly 2-layered at this stage.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		S1_S100a1 E15.5	Strong expression restricted to luminal cell layer of bladder epithelium from E14.5 onwards.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		S1_S100a1 E16.5	Strong expression restricted to luminal cell layer of bladder epithelium from E14.5 onwards.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		S1_S100a1 E17.5	Strong expression restricted to luminal cell layer of bladder epithelium from E14.5 onwards.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		S1_S100a1 E18.5	Strong expression restricted to luminal cell layer of bladder epithelium from E14.5 onwards.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		S1_S100a1 P7	Strong expression restricted to luminal cell layer of bladder epithelium from E14.5 onwards.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Strong		S1_S100a1 P14	Strong expression restricted to luminal cell layer of bladder epithelium from E14.5 onwards.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of bladder urothelium	bladder urothelial cell	Weak		S1_S100a1 P40	Strong expression restricted to luminal cell layer of bladder epithelium from E14.5 onwards.

Structure	Level	Pattern	Image	Note
TS22: urothelium of ureter	Absent		2_S100a1 E14.5

Structure	Level	Pattern	Image	Note
TS23: urothelium of ureter	Absent		2_S100a1 E15.5

Structure	Level	Pattern	Image	Note
TS24: urothelium of ureter	Absent		2_S100a1 E16.5

Structure	Cell Type	Level	Pattern	Image	Note
TS25: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	2_S100a1 E17.5	Expression first detected at E17.5 in some luminal cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: superficial cell layer of ureter	ureter urothelial cell	Present	Widespread	2_S100a1 E18.5	Expression in all luminal cells from E18.5 to P40.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	2_S100a1 P7	At postnatal stages, expression outlined arch-like shape of large luminal S-cells residing on top of a aggregation of I-cells and B-cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	2_S100a1 P14	At postnatal stages, expression outlined arch-like shape of large luminal S-cells residing on top of a aggregation of I-cells and B-cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: superficial cell layer of ureter	ureter urothelial cell	Present	Regionally restricted	2_S100a1 P40	At postnatal stages, expression outlined arch-like shape of large luminal S-cells residing on top of a aggregation of I-cells and B-cells.