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Gene Expression Data
Assay Details
Assay
Reference: J:219773 Francis SP, et al., A short splice form of Xin-actin binding repeat containing 2 (XIRP2) lacking the Xin repeats is required for maintenance of stereocilia morphology and hearing function. J Neurosci. 2015 Feb 4;35(5):1999-2014
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7407327
Gene symbol: Xirp2
Gene name: xin actin-binding repeat containing 2
Antibody: anti-XIRP2 (D-18)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3A E15: embryonic day 15.0 (more )
Note: Double labeled: green - Xirp2; red - actin.
Structure Cell Type Level Pattern Image Note
TS23: cochlea cochlea auditory hair cell Absent 3A E15 XIRP2 , 3A E15 Merge
Specimen 3A E18: embryonic day 18.0 (more )
Note: Double labeled: green - Xirp2; red - actin.
Structure Cell Type Level Pattern Image Note
TS26: cochlea cochlea auditory hair cell Present Regionally restricted 3A E18 Merge , 3A E18 XIRP2 Onset of expression in hair cells beginning at E18, coinciding with emergence of hair bundle.
Specimen 3A P4: postnatal day 4 (more )
Note: Double labeled: green - Xirp2; red - actin.
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlea auditory hair cell Present Regionally restricted 3A P4 Merge , 3A P4 XIRP2 Expression localized to apical region of hair cell and bundle.
Specimen 3A P10: postnatal day 10 (more )
Note: Double labeled: green - Xirp2; red - actin.
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlea auditory hair cell Present Regionally restricted 3A P10 XIRP2 , 3A P10 Merge Expression localized to apical region of hair cell and bundle.
Specimen 3A P20: postnatal day 20 (more )
Note: Double labeled: green - Xirp2; red - actin.
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlea auditory hair cell Present Regionally restricted 3A P20 Merge , 3A P20 XIRP2 Expression localized to apical region of hair cell and bundle.
Specimen 3B E18: embryonic day 18.0 (more )
Note: Double labeled: green - Xirp2; red - actin.
Structure Cell Type Level Pattern Image Note
TS26: utricle vestibular hair cell Present Regionally restricted 3B E18 Merge , 3B E18 XIRP2 Onset of expression at E18, specifically in apical regions of hair cells and stereocilia.
Specimen 3B P4: postnatal day 4 (more )
Note: Double labeled: green - Xirp2; red - actin.
Structure Cell Type Level Pattern Image Note
TS28: utricle vestibular hair cell Present Regionally restricted 3B P4 XIRP2 , 3B P4 Merge Expression specifically in apical regions of hair cells and stereocilia.
Specimen 3B P30: postnatal day 30 (more )
Note: Double labeled: green - Xirp2; red - actin.
Structure Cell Type Level Pattern Image Note
TS28: utricle vestibular hair cell Present Regionally restricted 3B P30 XIRP2 , 3B P30 Merge Expression is heterogeneous: strong expression in some stereocilia with weaker expression in stereocilia in same bundle.
Specimen 3C: postnatal day 4 (more )
Structure Cell Type Level Pattern Image Note
TS28: utricle vestibular hair cell Present Regionally restricted 3C XIRP2 Expression in vestibular hair bundle.
Specimen 8E WT: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: utricle Present Widespread 8E WT Merge , 8E WT XIRP2 Expression in all vestibular hair cells.
Specimen 9A D-18 WT utricle: postnatal day 4 (more )
Note: Triple labeled: blue - Xirp2 (D-18 antibody); green - Xirp2 (XRP2 minus exon 6 antibody; assay MGI:7407328); red - actin.
Structure Cell Type Level Pattern Image Note
TS28: utricle vestibular hair cell Present Regionally restricted 9A WT Utricle Merge , 9A WT Utricle D18 Expression in vestibular hair bundles.
Specimen 9A D-18 WT cochlea: postnatal day 4 (more )
Note: Triple labeled: blue - Xirp2 (D-18 antibody); green - Xirp2 (XRP2 minus exon 6 antibody; assay MGI:7407328); red - actin.
Structure Cell Type Level Pattern Image Note
TS28: cochlea cochlea auditory hair cell Present Regionally restricted 9A WT Cochlea D18 , 9A WT Cochlea Merge Expression in cochlear hair bundles.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory