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Gene Expression Data
Assay Details
Assay
Reference: J:330633 Shao A, et al., Multiomics analysis reveals that hepatocyte nuclear factor 1beta regulates axon guidance genes in the developing mouse kidney. Sci Rep. 2022 Oct 20;12(1):17586
Assay type: RNA in situ
MGI Accession ID: MGI:7388604
Gene symbol: Sema3d
Gene name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Probe: Mm-Sema3d (488111)
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope assay.
Results
Specimen 5c het: embryonic day 14.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hnf1btm3Mya/Hnf1b+, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. Double labeled: white - Sema3d; red - Cre reporter (labeling the ureteric bud).
Structure Level Pattern Image Note
TS22: collecting duct Strong 5c control Strong expression is seen in the ureteric bud.
TS22: mesenchyme of metanephros Weak 5c control Weak expression is seen in the metanephric mesenchyme.
Specimen 5c deficient: embryonic day 14.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Hnf1btm3Mya/Hnf1btm3Mya, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. Double labeled: white - Sema3d; red - Cre reporter (labeling the ureteric bud).
Structure Level Pattern Image Note
TS22: collecting duct Present 5c deficient Expression is seen in the ureteric bud; it is reduced in the mutant.
TS22: mesenchyme of metanephros Weak 5c deficient Weak expression is seen in the metanephric mesenchyme; it is unchanged in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory