Gene Expression Data
Assay Details
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Assay
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Reference:
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J:314181
Holzman MA, et al., HOXA5 Participates in Brown Adipose Tissue and Epaxial Skeletal Muscle Patterning and in Brown Adipocyte Differentiation. Front Cell Dev Biol. 2021;9:632303
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7384942
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Gene symbol:
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Plin1
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Gene name:
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perilipin 1
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Results
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Specimen
5C:
embryonic day 18.5
(more  )
Specimen
5C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: brown fat |
brown adipocyte |
Present |
Regionally restricted |
5C
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Expression in lipid droplets at periphery of adipocytes.
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Specimen
5D:
embryonic day 18.5
(more )
Note: Double labeled: magenta - Plin1; green - lipid dye
Specimen
5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Plin1; green - lipid dye
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: brown fat |
brown adipocyte |
Present |
Regionally restricted |
5D
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Expression in lipid droplets at periphery of adipocytes.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Hoxa5tm1Rob/Hoxa5tm1Rob
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: brown fat |
brown adipocyte |
Present |
Regionally restricted |
5H
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Lipid droplets are reduced in number and appear disorganized compared to control.
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Specimen
5I:
embryonic day 18.5; Hoxa5tm1Rob/ Hoxa5tm1Rob
(more )
Note: Double labeled: magenta - Plin1; green - lipid dye
Specimen
5I:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Hoxa5tm1Rob/Hoxa5tm1Rob
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Plin1; green - lipid dye
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: brown fat |
brown adipocyte |
Present |
Regionally restricted |
5I
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Lipid droplets are reduced in number and appear disorganized compared to control.
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Specimen
6D,H:
postnatal month 2; Hoxa5tm1Rob/Hoxa5 +
(more )
Specimen
6D,H:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Hoxa5tm1Rob/Hoxa5+
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Age:
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postnatal month 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brown fat |
brown adipocyte |
Present |
Regionally restricted |
6H
,
6D
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Expression outlining lipid droplets.
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Specimen
6I,M:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Hoxa5tm1Rob/Hoxa5tm1Rob
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Age:
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postnatal month 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brown fat |
brown adipocyte |
Present |
Regionally restricted |
6M
,
6I
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Lipid droplets were smaller, reduced in density and less organized compared to control.
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Analysis Tools