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Gene Expression Data
Assay Details
Assay
Reference: J:316543 Kim JS, et al., Reciprocal localization of transcription factors YY1 and CP2c in spermatogonial stem cells and their putative roles during spermatogenesis. Acta Histochem. 2016 Sep;118(7):685-692
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7384683
Gene symbol: Tfcp2
Gene name: transcription factor CP2
Antibody: Anti-LSF (clone 14; 610818)
Detection system: Secondary antibody coupled to alkaline phosphatase
Results
Specimen 3A Elongation stage: postnatal week 12 (more )
Note: Double labeled: blue - Tfcp2; brown - Yy1 (assay MGI:7384682).
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Present 3A , 3A'
TS28: spermatogonium Present 3A , 3A'
TS28: spermatid Absent 3A , 3A'
TS28: testis male germ line stem cell Absent 3A , 3A'

Specimen 3B Grouping stage: postnatal week 12 (more )
Note: Double labeled: blue - Tfcp2; brown - Yy1 (assay MGI:7384682).
Structure Level Pattern Image Note
TS28: spermatocyte Strong 3B , 3B'
TS28: spermatogonium Present 3B' , 3B
TS28: spermatid Present 3B' , 3B Staining intensity was lower in spermatids compared to spermagonia.

Specimen 3C Maturation stage: postnatal week 12 (more )
Note: Double labeled: blue - Tfcp2; brown - Yy1 (assay MGI:7384682).
Structure Cell Type Level Pattern Image Note
TS28: spermatid Present 3C' , 3C
TS28: spermatocyte Present 3C' , 3C
TS28: spermatogonium Present 3C' , 3C
TS28: spermatozoon Absent 3C' , 3C
TS28: testis male germ line stem cell Absent 3C' , 3C

Specimen 3D Release stage: postnatal week 12 (more )
Note: Double labeled: blue - Tfcp2; brown - Yy1 (assay MGI:7384682).
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Strong 3D' , 3D
TS28: spermatogonium Strong 3D' , 3D
TS28: spermatid Absent 3D' , 3D
TS28: spermatozoon Absent 3D' , 3D
TS28: testis male germ line stem cell Absent 3D , 3D'

Specimen 3E Elongation stage: postnatal week 12 (more )
Note: Double labeled: blue - Tfcp2; brown - Yy1 (assay MGI:7384682).
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Present 3E
TS28: spermatogonium Present 3E
TS28: spermatid Absent 3E
TS28: testis male germ line stem cell Absent 3E

Specimen 3F Grouping stage: postnatal week 12 (more )
Note: Double labeled: blue - Tfcp2; brown - Yy1 (assay MGI:7384682).
Structure Level Pattern Image Note
TS28: spermatocyte Strong 3F
TS28: spermatogonium Present 3F
TS28: spermatid Present 3F Staining intensity was lower in spermatids compared to spermagonia.

Specimen 3G Maturation stage: postnatal week 12 (more )
Note: Double labeled: blue - Tfcp2; brown - Yy1 (assay MGI:7384682).
Structure Cell Type Level Pattern Image Note
TS28: spermatid Present 3G
TS28: spermatocyte Present 3G
TS28: spermatogonium Present 3G
TS28: spermatozoon Absent 3G
TS28: testis male germ line stem cell Absent 3G

Specimen 3H Release stage: postnatal week 12 (more )
Note: Double labeled: blue - Tfcp2; brown - Yy1 (assay MGI:7384682).
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Strong 3H
TS28: spermatogonium Strong 3H
TS28: spermatid Absent 3H
TS28: spermatozoon Absent 3H
TS28: testis male germ line stem cell Absent 3H

Specimen Table 1 Elongation stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Absent
TS28: spermatogonium Strong
TS28: primary spermatocyte Moderate
TS28: spermatid Absent
TS28: spermatozoon Absent

Specimen Table 1 Grouping stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Absent
TS28: spermatogonium Weak
TS28: primary spermatocyte Moderate
TS28: spermatid Weak Non-Uniform
TS28: spermatozoon Absent

Specimen Table 1 Maturation stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Absent
TS28: spermatogonium Weak
TS28: primary spermatocyte Moderate
TS28: spermatid Weak
TS28: spermatozoon Absent

Specimen Table 1 Release stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Absent
TS28: spermatogonium Strong
TS28: primary spermatocyte Moderate
TS28: spermatid Absent
TS28: spermatozoon Absent


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory