About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:316543 Kim JS, et al., Reciprocal localization of transcription factors YY1 and CP2c in spermatogonial stem cells and their putative roles during spermatogenesis. Acta Histochem. 2016 Sep;118(7):685-692
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7384682
Gene symbol: Yy1
Gene name: YY1 transcription factor
Antibody: Anti-YY1 (EPR4652; ab109237)
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 3A Elongation stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Strong 3A' , 3A
TS28: spermatocyte Strong 3A , 3A'
TS28: spermatid Absent 3A' , 3A
TS28: spermatogonium Absent 3A' , 3A
Specimen 3B Grouping stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3B , 3B'
TS28: spermatid Present 3B' , 3B
TS28: spermatocyte Present 3B' , 3B
TS28: spermatogonium Present 3B' , 3B
TS28: spermatozoon Absent 3B , 3B'
TS28: seminiferous tubule Present Non-Uniform 3B' , 3B Intensity of YY1 staining progressively decreased from spermatogonial stem cell through spermatocyte, spermatid, and spermatogonia.
Specimen 3C Maturation stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3C' , 3C
TS28: spermatocyte Present 3C' , 3C
TS28: spermatogonium Present 3C' , 3C
TS28: spermatozoon Absent 3C' , 3C
Specimen 3D Release stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3D , 3D'
TS28: spermatocyte Present 3D , 3D'
TS28: spermatogonium Weak 3D , 3D'
TS28: spermatid Absent 3D' , 3D
TS28: spermatozoon Absent 3D' , 3D
Specimen 3E Elongation stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Strong 3E
TS28: spermatocyte Strong 3E
TS28: spermatid Absent 3E
TS28: spermatogonium Absent 3E
Specimen 3F Grouping stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3F
TS28: spermatid Present 3F
TS28: spermatocyte Present 3F
TS28: spermatogonium Present 3F
TS28: spermatozoon Absent 3F
TS28: seminiferous tubule Present Non-Uniform 3F Intensity of YY1 staining progressively decreased from spermatogonial stem cells through spermatocyte, spermatid, and spermatogonia.
Specimen 3G Maturation stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3G
TS28: spermatocyte Present 3G
TS28: spermatogonium Present 3G
TS28: spermatozoon Absent 3G
Specimen 3H Release stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3H
TS28: spermatocyte Present 3H
TS28: spermatogonium Weak 3H
TS28: spermatid Absent 3H
TS28: spermatozoon Absent 3H
Specimen Table 1 Elongation stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Moderate
TS28: spermatogonium Absent
TS28: primary spermatocyte Strong
TS28: spermatid Absent
TS28: spermatozoon Absent
Specimen Table 1 Grouping stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Strong
TS28: spermatogonium Weak
TS28: primary spermatocyte Strong
TS28: spermatid Moderate
TS28: spermatozoon Absent
Specimen Table 1 Maturation stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Moderate
TS28: spermatogonium Moderate
TS28: primary spermatocyte Strong
TS28: spermatid Weak
TS28: spermatozoon Absent
Specimen Table 1 Release stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Moderate
TS28: spermatogonium Weak Non-Uniform
TS28: primary spermatocyte Strong
TS28: spermatid Absent
TS28: spermatozoon Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory