Akt2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:47692 Altomare DA, et al., Akt2 mRNA is highly expressed in embryonic brown fat and the AKT2 kinase is activated by insulin. Oncogene. 1998 May 7;16(18):2407-11
Assay type:	RNA in situ
MGI Accession ID:	MGI:7384573
Gene symbol:	Akt2
Gene name:	thymoma viral proto-oncogene 2

Probe:	Akt2 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Results

Specimen 1B: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: liver	Strong		1B
TS22: skeletal muscle	Strong		1B

Specimen 1F: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: liver	Strong		1F
TS24: seminiferous cord	Strong		1F
TS24: skeletal muscle	Strong		1F
TS24: brown fat	Strong	Regionally restricted	1F	Expression is in brown fat of of the cervical region.
TS24: central nervous system	Weak		1F
TS24: cervical ganglion	Weak		1F

Specimen 1H: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: liver	Strong		1H
TS24: skeletal muscle	Strong		1H
TS24: brown fat	Very strong	Regionally restricted	1H	Expression is in brown fat of of the cervical region.

Specimen 2B: postnatal adult (more

)

Structure	Level	Image
TS28: cerebellum Purkinje cell layer	Strong	2B
TS28: cerebellum granule cell layer	Present	2B
TS28: cerebral cortex	Weak
TS28: hippocampus	Weak
TS28: thalamus	Weak

Specimen not shown E11.5: embryonic day 11.5 (more

)

Specimen not shown E15.5: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: liver	Strong
TS23: skeletal muscle	Strong

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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