Srf Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:50876 Arsenian S, et al., Serum response factor is essential for mesoderm formation during mouse embryogenesis. EMBO J. 1998 Nov 2;17(21):6289-99
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7384338
Gene symbol:	Srf
Gene name:	serum response factor

Antibody:	anti-Srf
Assay notes:	Specimens were fixed in either 4% paraformaldehyde or in an aqueous solution containing 35% methanol, 35% acetone and 5% acetic acid.

Results

Specimen 1A: embryonic day 6.5 (more

)

Structure	Level	Image
TS9: embryo ectoderm	Present	1A
TS9: embryo endoderm	Present	1A
TS9: extraembryonic ectoderm	Present	1A
TS9: extraembryonic endoderm	Present	1A

Specimen 1B: embryonic day 7.5 (more

)

Structure	Level	Image
TS11: embryo ectoderm	Present	1B
TS11: embryo endoderm	Present	1B
TS11: embryo mesoderm	Present	1B
TS11: extraembryonic ectoderm	Present	1B
TS11: extraembryonic endoderm	Present	1B
TS11: extraembryonic mesoderm	Present	1B

Specimen 1C: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Strong		1D , 1C
TS13: embryo	Present	Regionally restricted	1D , 1C	Expression is in the heart and is barely detectable in other tissues.
TS13: somite	Absent		1D , 1C

Specimen 1E: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: cardiac muscle tissue	Strong		1E

Specimen 1F: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: myotome	Present		1F

Specimen 5E: embryonic day 6.5; Srf^tm1Nor/Srf^tm1Nor (more

)

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent		5E

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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