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Gene Expression Data
Assay Details
Assay
Reference: J:322165 Cunningham JG, et al., Early construction of the thalamocortical axon pathway requires c-Jun N-terminal kinase signaling within the ventral forebrain. Dev Dyn. 2022 Mar;251(3):459-480
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7382847
Gene symbol: Ntng1
Gene name: netrin G1
Antibody: Netrin-G1a Antibody (AF1166)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 2D: embryonic day 15.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex Present 2D
TS23: cerebral internal capsule Present 2D
TS23: forebrain Present Regionally restricted 2D Positive thalamocortical axons crossed the diencephalon-telencephalon boundary, extended through the internal capsule, and reached the mid-cortical wall.
Specimen 2E: embryonic day 15.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Structure Level Pattern Image Note
TS23: forebrain Present Regionally restricted 2E Positive thalamocortical axons crossed the diencephalon-telencephalon boundary, extended through the internal capsule, and reached the mid-cortical wall.
TS23: cerebral cortex Present 2E
TS23: cerebral internal capsule Present 2E
Specimen 2F: embryonic day 15.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex Present 2F
TS23: cerebral internal capsule Present 2F
TS23: forebrain Present Regionally restricted 2F Positive thalamocortical axons crossed the diencephalon-telencephalon boundary, extended through the internal capsule, and reached the mid-cortical wall.
Specimen 2G: embryonic day 15.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex Present 2G
TS23: cerebral internal capsule Present 2G
TS23: forebrain Present Regionally restricted 2G Positive thalamocortical axons crossed the diencephalon-telencephalon boundary, extended through the internal capsule, and reached the mid-cortical wall.
Specimen 2H: embryonic day 15.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex Present 2H
TS23: cerebral internal capsule Present 2H
TS23: forebrain Present Regionally restricted 2H Positive thalamocortical axons crossed the diencephalon-telencephalon boundary, extended through the internal capsule, and reached the mid-cortical wall.
Specimen 2I: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: telencephalon Present Regionally restricted 2I Positive fibers aberrantly projected rostrally along the ventromedial margin.
Specimen 2J: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: telencephalon Present Regionally restricted 2J Positive fibers aberrantly projected rostrally along the ventromedial margin.
TS23: corpus striatum Present 2J Positive fibers aberrantly projected into the striatum at ectopic locations.
Specimen 2K: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: hypothalamus Present 2K Positive fibers aberrantly projected ventrally into the hypothalamus.
TS23: telencephalon Present Regionally restricted 2K Positive fibers aberrantly projected rostrally along the ventromedial margin.
Specimen 2L: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: hypothalamus Present 2L Positive fibers aberrantly projected ventrally into the hypothalamus.
TS23: telencephalon Present Regionally restricted 2L Positive fibers aberrantly projected rostrally along the ventromedial margin.
Specimen 2M: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: hypothalamus Present 2M Positive fibers aberrantly projected ventrally into the hypothalamus.
TS23: telencephalon Present Regionally restricted 2M Positive fibers aberrantly projected rostrally along the ventromedial margin.
Specimen 2O: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: hypothalamus Present 2O Positive fibers aberrantly projected ventrally into the hypothalamus.
TS23: telencephalon Present Regionally restricted 2O Positive fibers aberrantly projected rostrally along the ventromedial margin, avoided entry into the ventral telencephalon completely.
Specimen 2P: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: hypothalamus Present 2P Positive fibers aberrantly projected ventrally into the hypothalamus.
TS23: telencephalon Present Regionally restricted 2P Positive fibers aberrantly projected rostrally along the ventromedial margin, but failed to reach the internal capsule.
Specimen 2Q: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: hypothalamus Present 2Q Positive fibers aberrantly projected ventrally into the hypothalamus.
TS23: telencephalon Present Regionally restricted 2Q Positive fibers aberrantly projected rostrally along the ventromedial margin, reached a rudimentary internal capsule but did not cross the pallial-subpallial boundary.
Specimen 2R: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: hypothalamus Present 2R Positive fibers aberrantly projected ventrally into the hypothalamus.
TS23: telencephalon Present Regionally restricted 2R Positive fibers aberrantly projected rostrally along the ventromedial margin, reached the cortical rudiment, however not to the same extent as controls.
Specimen 5A: embryonic day 12.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Structure Level Pattern Image Note
TS20: telencephalon Present Regionally restricted 5A Positive thalamocortical axons extend into the ventral telencephalon.
Specimen 5B: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted 5B Positive thalamocortical axons reached the pallial-subpallial boundary.
Specimen 5C: embryonic day 15.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex Present 5C Positive thalamocortical axons continued to extend through the internal capsule and into the cortex.
TS23: cerebral internal capsule Present 5C Positive thalamocortical axons continued to extend through the internal capsule and into the cortex.
Specimen 5D: embryonic day 17.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Structure Level Pattern Image Note
TS25: cerebral cortex Present 5D Positive thalamocortical axons continued to extend through the internal capsule and into the cortex.
TS25: cerebral internal capsule Present 5D Positive thalamocortical axons continued to extend through the internal capsule and into the cortex.
Specimen 5E: postnatal day 0; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Structure Level Pattern Image Note
TS27: cerebral cortex Present 5E Positive thalamocortical axons continued to extend through the internal capsule and into the cortex.
TS27: cerebral internal capsule Present 5E Positive thalamocortical axons continued to extend through the internal capsule and into the cortex.
Specimen 5F: embryonic day 12.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: telencephalon Absent 5F
Specimen 5G: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: telencephalon Absent 5G
Specimen 5H: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: forebrain Present Regionally restricted 5H Positive thalamocortical axons were present adjacent to the diencephalon-telencephalon boundary or below the hypothalamus.
Specimen 5I: embryonic day 17.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: forebrain Present Regionally restricted 5I Positive thalamocortical axons were present adjacent to the diencephalon-telencephalon boundary or below the hypothalamus.
Specimen 5J: postnatal day 0; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: forebrain Present Regionally restricted 5J Positive thalamocortical axons were present adjacent to the diencephalon-telencephalon boundary or below the hypothalamus.
Specimen 5K/L: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Ntng1; green -Cre reporter.
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 5L , 5K Positive thalamocortical axons project from the thalamus and cross into the ventral telencephalon at the diencephalon-telencephalon boundary.
Specimen 5M/N: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Ntng1; green - Cre reporter.
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted 5N , 5M Positive thalamocortical axons have extended from the thalamus; however, they have not crossed the diencephalon-telencephalon boundary.
Specimen 9B: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Ntng1; green - Th (assay MGI:7382856).
Structure Level Pattern Image Note
TS21: forebrain Present 9B Positive thalamocortical axons crossed the diencephalon-telencephalon boundary and enter the telencephalon.
TS21: diencephalon Present 9B
TS21: telencephalon Present 9B
Specimen 9C: embryonic day 13.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Ntng1; green - Th (assay MGI:7382856).
Structure Level Pattern Image Note
TS21: forebrain Present 9C Positive thalamocortical axons crossed the diencephalon-telencephalon boundary and enter the telencephalon.
TS21: diencephalon Present 9C
TS21: telencephalon Present 9C
Specimen 9E: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Ntng1; green - Th (assay MGI:7382856).
Structure Level Pattern Image Note
TS21: diencephalon Present 9E
TS21: telencephalon Absent 9E Axons failed to reach the telencephalon.
Specimen 9F: embryonic day 13.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Ntng1; green - Th (assay MGI:7382856).
Structure Level Pattern Image Note
TS21: diencephalon Present 9F
TS21: telencephalon Absent 9F Axons failed to reach the telencephalon.
Specimen 9G: embryonic day 15.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Ntng1; green - Th (assay MGI:7382856).
Structure Level Pattern Image Note
TS23: cerebral cortex Present 9G
TS23: diencephalon Present 9G
Specimen 9H: embryonic day 15.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Ntng1; green - Th (assay MGI:7382856).
Structure Level Pattern Image Note
TS23: cerebral cortex Present 9H
TS23: diencephalon Present 9H
Specimen 9I: embryonic day 15.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Ntng1; green - Th (assay MGI:7382856).
Structure Level Pattern Image Note
TS23: diencephalon Present 9I
Specimen 9J: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Ntng1; green - Th (assay MGI:7382856).
Structure Level Pattern Image Note
TS23: cerebral cortex Absent 9J
TS23: diencephalon Present 9J
Specimen 9K: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Ntng1; green - Th (assay MGI:7382856).
Structure Level Pattern Image Note
TS23: diencephalon Present 9K Positive thalamocortical axons misproject ventrally.
TS23: cerebral cortex Absent 9K Axons fail to reach the telencephalon.
Specimen 9L: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Ntng1; green - Th (assay MGI:7382856).
Structure Level Pattern Image Note
TS23: diencephalon Present 9L Positive thalamocortical axons misproject ventrally.
TS23: cerebral cortex Absent 9L Axons fail to reach the telencephalon.
Specimen 10A: embryonic day 15.5; Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Ntng1; green - Cre reporter.
Structure Level Pattern Image Note
TS23: cerebral cortex Present 10A
TS23: cerebral internal capsule Present 10A
Specimen 10B: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Ntng1; green - Cre reporter.
Structure Level Pattern Image Note
TS23: diencephalon Present Regionally restricted 10B Positive axons projected to the ventromedial margin of the telencephalon and extended into the hypothalamus.
Specimen 10C: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Double labeled: red - Ntng1; green - Cre reporter.
Structure Level Pattern Image Note
TS23: cerebral cortex Present 10C Similar as in the wild-type control.
TS23: cerebral internal capsule Present 10C Similar as in the wild-type control.
Specimen 10D: embryonic day 15.5; Mapk8tm1Rjd/Mapk8tm1Rjd, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Ntng1; green - Cre reporter.
Structure Level Pattern Image Note
TS23: cerebral cortex Present 10D Similar as in the wild-type control.
TS23: cerebral internal capsule Present 10D Similar as in the wild-type control.
Specimen 10E: embryonic day 15.5; Mapk10tm1Flv/Mapk10tm1Flv, Mapk8tm1Rjd/Mapk8+, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Ntng1; green - Cre reporter.
Structure Level Pattern Image Note
TS23: cerebral cortex Present 10E Similar as in the wild-type control.
TS23: cerebral internal capsule Present 10E Similar as in the wild-type control.
Specimen 10F: embryonic day 15.5; Mapk10tm1Flv/Mapk10+, Mapk8tm1Rjd/Mapk8tm1Rjd, Mapk9tm1Flv/Mapk9tm1Flv, Tg(mI56i-cre,EGFP)1Kc/0 (more )
Note: Conditional mutant. Double labeled: red - Ntng1; green - Cre reporter.
Structure Level Pattern Image Note
TS23: cerebral internal capsule Present 10F Similar as in the wild-type control.
TS23: cerebral cortex Present 10F Shorter length of the medial extension into the cortex compared with the wild-type control.
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03/18/2025
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