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Gene Expression Data
Assay Details
Assay
Reference: J:186260 Yoshida A, et al., Localization of septin proteins in the mouse cochlea. Hear Res. 2012 Jul;289(1-2):40-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7380406
Gene symbol: Septin7
Gene name: septin 7
Antibody: anti-SEPT7/CDC10
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5a/b: postnatal adult (more )
Note: Double labeled: green - Septin7; red - F-actin.
Structure Cell Type Level Pattern Image Note
TS28: organ of Corti external pillar cell of cochlea Strong 5a , 5b
TS28: organ of Corti internal pillar cell of cochlea Strong 5a , 5b
TS28: organ of Corti Deiter's cell Present Regionally restricted 5b , 5a Expression in filament-like structure between reticular lamina and basilar membrane lateral to outer pillar cells - co-expressed with F-actin.
TS28: organ of Corti cochlea auditory hair cell Weak 5b , 5a
TS28: organ of Corti Hensen cell Weak 5b , 5a
TS28: organ of Corti organ of Corti supporting cell Weak 5b , 5a
TS28: external spiral sulcus Weak Regionally restricted 5b , 5a Weaker expression in inner and outer sulcus cells.
TS28: internal spiral sulcus Weak Regionally restricted 5b , 5a Weaker expression in inner and outer sulcus cells.

Specimen 5d/f: postnatal adult (more )
Note: Double labeled: green - Septin7; red - Tubb3 (assay MGI:7380402).
Structure Level Pattern Image Note
TS28: organ of Corti Present Regionally restricted 5f , 5d Co-expression with Tubb3 in punctate structures but not in filament-like structure.

Specimen 5g/i: postnatal adult (more )
Note: Double labeled: green - Septin7; red - Syp (assay MGI:7380405).
Structure Level Pattern Image Note
TS28: organ of Corti Present Regionally restricted 5g inset , 5i inset` , 5g , 5i Co-expression with synaptophysin in punctate structure below inner hair cells and around basal portion of outer hair cells.

Specimen 5j/l: postnatal adult (more )
Note: Double labeled: green - Septin7; red - acetylated tubulin.
Structure Cell Type Level Pattern Image Note
TS28: organ of Corti Deiter's cell Present Regionally restricted 5l , 5j Expression in filament-like structure co-expressed with acetylated alpha tubulin.

Specimen 6c/f: postnatal adult (more )
Note: Double labeled: green - Septin7; red - Myo7a (assay MGI:7380409).
Structure Cell Type Level Pattern Image Note
TS28: organ of Corti Deiter's cell Present Regionally restricted 6c , 6f Expression in filament-like structures originating from basilar membrane and extending to apices of outer hair cells, running outside of outer hair cells. Expression in phalangeal process of Deiters' cells.

Specimen 8d/f: postnatal adult; Septin5tm1Wtr/Septin5tm1Wtr (more )
Note: Double labeled: green - Septin7; red - Syp (assay MGI:7380405).
Structure Cell Type Level Pattern Image Note
TS28: organ of Corti Deiter's cell Present 8f , 8d Expression pattern similar to that of wild type cochlea in pillar cells, Deiters' cells and presynaptic vesicles.
TS28: organ of Corti pillar cell Present 8f , 8d Expression pattern similar to that of wild type cochlea in pillar cells, Deiters' cells and presynaptic vesicles.

Specimen 10a/c: embryonic day 13.0 (more )
Note: Double labeled: green - Septin7; red - F-actin.
Structure Level Pattern Image Note
TS21: cochlear duct epithelium Present Widespread 10a , 10c Expression in most epithelial cells of cochlea duct.

Specimen 10d/f: embryonic day 15.0 (more )
Note: Double labeled: green - Septin7; red - F-actin.
Structure Level Pattern Image Note
TS23: cochlear duct epithelium Strong Widespread 10d , 10f Similar expression maintained until E15 with more intense expression level.

Specimen 10g/i: postnatal day 1 (more )
Note: Double labeled: green - Septin7; red - F-actin.
Structure Cell Type Level Pattern Image Note
TS27: organ of Corti pillar cell Very strong Regionally restricted 10i , 10g Intense expression in cytoplasm of pillar cells.
TS27: organ of Corti Deiter's cell Present 10g , 10i
TS27: organ of Corti Hensen cell Present 10g , 10i
TS27: cochlea Claudius cell Present 10g , 10i

Specimen 10j/l: postnatal day 2 (more )
Note: Double labeled: green - Septin7; red - F-actin.
Structure Cell Type Level Pattern Image Note
TS27: organ of Corti pillar cell Strong Regionally restricted 10j , 10l Stronger expression at apical portion of pillar cells through P2 and P3.

Specimen 10m/o: postnatal day 3 (more )
Note: Double labeled: green - Septin7; red - F-actin.
Structure Cell Type Level Pattern Image Note
TS27: organ of Corti Deiter's cell Strong 10m , 10o Stronger expression than in other epithelial cells in pillar cells, Deiters' cells, Hensen't cells and Claudius cells.
TS27: organ of Corti Hensen cell Strong 10m , 10o Stronger expression than in other epithelial cells in pillar cells, Deiters' cells, Hensen't cells and Claudius cells.
TS27: organ of Corti pillar cell Strong 10m , 10o Stronger expression than in other epithelial cells in pillar cells, Deiters' cells, Hensen't cells and Claudius cells.
TS27: cochlea Claudius cell Strong 10m , 10o

Specimen 10p/r: postnatal day 7 (more )
Note: Double labeled: green - Septin7; red - F-actin.
Structure Level Pattern Image Note
TS28: cochlea Present Regionally restricted 10r , 10p Expression pattern similar to that in adult cochlea. No expression in presynaptic vesicles.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory