Gene Expression Data
Assay Details
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Assay
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Reference:
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J:186260
Yoshida A, et al., Localization of septin proteins in the mouse cochlea. Hear Res. 2012 Jul;289(1-2):40-51
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7380400
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Gene symbol:
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Septin4
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Gene name:
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septin 4
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Antibody:
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anti-SEPT4/H5C2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3a/c:
postnatal adult
(more  )
Note: Double labeled: green - Septin4; blue - F-actin.
Specimen
3a/c:
(close  )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin4; blue - F-actin.
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Specimen
3e:
postnatal adult
(more )
Note: Double labeled: green - Septin4; red - Tubb3 (assay MGI:7380402).
Specimen
3e:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin4; red - Tubb3 (assay MGI:7380402).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
Present |
Regionally restricted |
3e
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No overlap with Tuj1 (Tubb3) expression in nerve fibers.
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Specimen
3f:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Septin4tm1Ksh/Septin4tm1Ksh
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3g/i:
postnatal adult
(more )
Note: Double labeled: green - Septin4; red - acetylated tubulin.
Specimen
3g/i:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin4; red - acetylated tubulin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Deiter's cell |
Strong |
Regionally restricted |
3i
,
3g
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Expression overlapped with that of acetylated alpha tubulin, abundant in Deiters' cells.
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Specimen
6a/d:
postnatal adult
(more )
Note: Double labeled: green - Septin4; red - Myo7a (assay MGI:7380409).
Specimen
6a/d:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin4; red - Myo7a (assay MGI:7380409).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Deiter's cell |
Present |
Regionally restricted |
6a
,
6d
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Expression in filament-like structures originating from basilar membrane and extending to apices of outer hair cells, running outside of outer hair cells. Expression in phalangeal process of Deiters' cells.
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Specimen
8a/c:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Septin5tm1Wtr/Septin5tm1Wtr
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin4; red - Syp (assay MGI:7380405).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Deiter's cell |
Present |
Regionally restricted |
8c
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8a
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Expression similar to that of wild type cochlea. Expression did not overlap with that of synaptophysin
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Specimen
E13 not shown:
embryonic day 13.0
(more )
Specimen
E13 not shown:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cochlea |
Absent |
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No expression in cochlea through P3.
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Specimen
E15 not shown:
embryonic day 15.0
(more )
Specimen
E15 not shown:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea |
Absent |
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No expression in cochlea through P3.
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Specimen
P1 not shown:
postnatal day 1
(more )
Specimen
P1 not shown:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Absent |
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No expression in cochlea through P3.
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Specimen
P2 not shown:
postnatal day 2
(more )
Specimen
P2 not shown:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Absent |
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No expression in cochlea through P3.
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Specimen
9a/c:
postnatal day 3
(more )
Note: Double labeled: green - Septin4; red - F-actin.
Specimen
9a/c:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin4; red - F-actin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Deiter's cell |
Present |
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9c
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9a
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Expression first detected in outer pillar cells and Deiters' cells.
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| TS27: organ of Corti |
external pillar cell of cochlea |
Present |
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9c
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9a
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Expression first detected in outer pillar cells and Deiters' cells.
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| TS27: organ of Corti |
cochlear inner hair cell |
Weak |
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9c
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9a
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| TS27: organ of Corti |
cochlear outer hair cell |
Strong |
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9a
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9c
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Specimen
9d/f:
postnatal day 7
(more )
Note: Double labeled: green - Septin4; red - F-actin.
Specimen
9d/f:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Septin4; red - F-actin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Deiter's cell |
Present |
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9d
,
9f
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Expression first detected in outer pillar cells and Deiters' cells.
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| TS28: organ of Corti |
external pillar cell of cochlea |
Present |
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9d
,
9f
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Expression first detected in outer pillar cells and Deiters' cells.
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| TS28: organ of Corti |
cochlear inner hair cell |
Weak |
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9d
,
9f
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| TS28: organ of Corti |
cochlear outer hair cell |
Strong |
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9f
,
9d
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