Gene Expression Data
Assay Details
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Assay
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Reference:
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J:309724
Rux D, et al., Hox11 expression characterizes developing zeugopod synovial joints and is coupled to postnatal articular cartilage morphogenesis into functional zones in mice. Dev Biol. 2021 Sep;477:49-63
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7380285
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Gene symbol:
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Hoxa11
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Gene name:
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homeobox A11
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Reporter:
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GFP
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Direct Detection
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Assay notes:
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Multi-photon microscopy to image and produce 3D reconstructions of the live Hoxa11eGFP reporter in combination with second harmonic generation (SHG) to visualize collagen.
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Results
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Specimen
5I:
(close  )
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Genetic Background:
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B6.129-Hoxa11tm1Dmwe/J
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Mutant Allele(s):
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Hoxa11tm1Dmwe/Hoxa11+
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Age:
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postnatal month 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: articular cartilage |
chondrocyte |
Present |
Regionally restricted |
5I
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Expression was detected in articular cartilage of the tibia. Top view showed that Hoxa11eGFP expression characterized the entire chondrocyte columns flanked by similarly-oriented collagen fibers.
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Specimen
5J:
(close )
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Genetic Background:
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B6.129-Hoxa11tm1Dmwe/J
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Mutant Allele(s):
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Hoxa11tm1Dmwe/Hoxa11+
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Age:
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postnatal month 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: articular cartilage |
chondrocyte |
Present |
Regionally restricted |
5J
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Expression was detected in articular cartilage of the tibia. Side view showed that Hoxa11eGFP expression characterized the entire chondrocyte columns flanked by similarly-oriented collagen fibers.
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Analysis Tools
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