Probe:	Mm-Hoxa11 (495561)
Probe preparation:	Antisense
Visualized with:	Fluorescence
Assay notes:	RNAscope was performed using the RED2.5 detection system.

Structure	Level	Pattern	Image	Note
TS23: forelimb	Present	Regionally restricted	7A	High expression was noted in the distal zeugopod surrounding and including the developing distal growth plates. Highest expression was restricted to distal end of both zeugopod bones and most proximal wrist bones.
TS23: zeugopod	Present	Regionally restricted	7A	High expression was noted in the distal zeugopod surrounding and including the developing distal growth plates. Highest expression was restricted to distal end of both zeugopod bones and most proximal wrist bones.
TS23: scapholunate	Present		7A

Structure	Level	Pattern	Image	Note
TS27: wrist	Present	Widespread	7D	High expression was detected broadly in all elements of the newborn wrist.

Structure	Level	Pattern	Image	Note
TS27: elbow	Weak		7F

Structure	Level	Pattern	Image	Note
TS28: wrist	Present	Regionally restricted	7H	Expression was maintained in wrist cartilage in the adult.

Structure	Level	Pattern	Image	Note
TS28: synovium	Present	Regionally restricted	7J	Expression was detected in the wrist synovium.

Structure	Level	Pattern	Image	Note
TS23: calcaneus	Present		8A
TS23: talus	Present		8A
TS23: tibia	Present	Regionally restricted	8A	High expression was detected in distal tibia.
TS23: ankle	Present	Regionally restricted	8A	Expression was detected in the developing achilles tendon.
TS23: knee	Present		8A

Structure	Level	Pattern	Image	Note
TS27: ankle	Present	Widespread	8D	The expression patterns from E15.5 were maintained at birth.
TS27: tendo calcaneus	Present		8D

Structure	Level	Pattern	Image	Note
TS27: knee joint	Present	Regionally restricted	8G Hoxa11	High expression was detected in the medial region.

Structure	Level	Pattern	Image	Note
TS27: knee joint	Present	Regionally restricted	8H Hoxa11	High expression was detected in the center region.

Structure	Level	Pattern	Image	Note
TS27: knee joint	Weak		8I Hoxa11

Structure	Level	Pattern	Image	Note
TS27: patella	Weak		8J Hoxa11

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present		8K Hoxa11

Structure	Cell Type	Level	Pattern	Image	Note
TS28: synovium	type B synovial cell	Present		8L Hoxa11

Structure	Level	Pattern	Image	Note
TS28: knee joint	Present	Regionally restricted	8M Hoxa11	Expression was detected in cartilage.

Structure	Level	Pattern	Image	Note
TS28: ligament	Present	Regionally restricted	8N Hoxa11	Expression was detected in the knee ligament.

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present	Regionally restricted	8O Hoxa11	Expression was detected in the tibia bone marrow.

Structure	Level	Pattern	Image	Note
TS23: shoulder	Weak		S5A

Structure	Level	Pattern	Image	Note
TS23: hip	Weak		S5D

Structure	Level	Pattern	Image	Note
TS23: sacral vertebral cartilage condensation	Present		S5G