Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:76349
Hussain MA, et al., Brn-4 Transcription Factor Expression Targeted to the Early Developing Mouse Pancreas Induces Ectopic Glucagon Gene Expression in Insulin-producing beta Cells. J Biol Chem. 2002 May 3;277(18):16028-32
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7378428
|
|
Gene symbol:
|
Pou3f4
|
|
Gene name:
|
POU domain, class 3, transcription factor 4
|
|
Antibody:
|
anti-Brn4 (4R2 )
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
|
Results
|
Specimen
1C WT Brn-4:
embryonic day 18.0
(more  )
Specimen
1C WT Brn-4:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: islets of Langerhans |
pancreatic A cell |
Present |
Regionally restricted |
1C WT Brn-4
|
Expression restricted to alpha cells located at periphery of islets.
|
|
Analysis Tools
