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Gene Expression Data
Assay Details
Assay
Reference: J:186532 Mitchell DC, et al., GEFT, A Rho family guanine nucleotide exchange factor, regulates lens differentiation through a Rac1-mediated mechanism. Curr Mol Med. 2011 Aug;11(6):465-80
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7378195
Gene symbol: Arhgef25
Gene name: Rho guanine nucleotide exchange factor 25
Antibody: Anti-GEFT
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 2A: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: optic vesicle neural ectoderm Present Regionally restricted 2A Expression was detected in neuroepithelium adjacent to the lumen of the optic vesicle.
TS14: eye mesenchyme Present Regionally restricted 2A Expression was detected in mesenchyme adjacent to the optic vesicle.

Specimen 2B: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: lens fiber Strong 2B , 2C
TS25: lens epithelium Present Regionally restricted 2C , 2B Expression was detected in the cuboidal epithelium of the lens.
TS25: eyelid epithelium Present 2C , 2B
TS25: eyelid mesenchyme Present Regionally restricted 2B , 2C Expression was detected in the endothelial cell layer of the developing eyelid.
TS25: neural retina Present Regionally restricted 2D , 2B Elevated expression was detected in the neuroblastic layer of the developing retina.

Specimen 3A: postnatal (more )
Structure Cell Type Level Pattern Image Note
TS28: cornea epithelium stratified squamous epithelial cell Strong Regionally restricted 3Aa , 3Ac , 3Ab Expression was highly detected in the squamous cells of the nonpapillated, stratified squamous non-keratinized corneal epithelium.
TS28: cornea epithelium columnar/cuboidal epithelial cell Strong 3Ac , 3Ab , 3Aa
TS28: cornea endothelium Weak 3Ac , 3Ab , 3Aa
TS28: cornea stroma Weak 3Ac , 3Ab , 3Aa

Specimen 3B: postnatal (more )
Structure Cell Type Level Pattern Image Note
TS28: lens epithelium lens epithelial cell Strong 3Bb , 3Bc , 3Ba
TS28: lens fiber Weak 3Ba , 3Bb , 3Bc

Specimen 3C: postnatal (more )
Structure Cell Type Level Pattern Image Note
TS28: retina ganglion cell layer retinal ganglion cell Present 3Ca , 3Cc , 3Cb Expression was detected in the axons and cell bodies of the retinal ganglion cells. The highest retinal expression was in the ganglion cell layer.
TS28: retina inner plexiform layer Present Regionally restricted 3Cc , 3Ca , 3Cb Expression was detected in the nerve fibers.
TS28: retina outer plexiform layer Present Regionally restricted 3Cc , 3Ca , 3Cb Expression was detected in the nerve fibers.
TS28: photoreceptor layer Present 3Ca , 3Cc , 3Cb
TS28: retina outer nuclear layer retinal cone cell Weak 3Ca , 3Cc , 3Cb
TS28: retina outer nuclear layer retinal rod cell Weak 3Ca , 3Cc , 3Cb
TS28: retina inner nuclear layer amacrine cell Moderate 3Cc , 3Cb , 3Ca Expression was in nuclei.
TS28: retina inner nuclear layer Mueller cell Moderate 3Cc , 3Cb , 3Ca Expression was in nuclei.
TS28: retina inner nuclear layer retina horizontal cell Moderate 3Cc , 3Cb , 3Ca Expression was in nuclei.
TS28: retina inner nuclear layer retinal bipolar neuron Moderate 3Cc , 3Cb , 3Ca Expression was in nuclei.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory