Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:137010
Petrou P, et al., Basement membrane distortions impair lung lobation and capillary organization in the mouse model for fraser syndrome. J Biol Chem. 2005 Mar 18;280(11):10350-6
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7377699
|
|
Gene symbol:
|
Agrn
|
|
Gene name:
|
agrin
|
|
Antibody:
|
anti-Agrin
|
|
Detection system:
|
Secondary antibody
|
|
|
Results
|
Specimen
3C:
embryonic day 14.5
(more  )
Note: Specimen was the dorsal part of the left lung.
Specimen
3C:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Specimen was the dorsal part of the left lung.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Present |
Regionally restricted |
3C
|
Expression is in the basement membrane of the visceral pleura and conducting airways.
|
| TS22: visceral pleura |
Present |
Regionally restricted |
3C
|
Expression is in the basement membrane of the visceral pleura and conducting airways.
|
Specimen
3D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * NMRI
|
|
Mutant Allele(s):
|
Fras1tm1Chpk/Fras1tm1Chpk
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Specimen was the dorsal part of the left lung.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Weak |
Regionally restricted |
3D
|
Expression is in the basement membrane of the visceral pleura and conducting airways. The reduction was more pronounced in the visceral pleura basement membrane.
|
| TS22: visceral pleura |
Weak |
Regionally restricted |
3D
|
Expression is in the basement membrane of the visceral pleura and conducting airways. The reduction was more pronounced in the visceral pleura basement membrane.
|
Specimen
3G:
embryonic day 14.5
(more )
Specimen
3G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
3H:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * NMRI
|
|
Mutant Allele(s):
|
Fras1tm1Chpk/Fras1tm1Chpk
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Present |
Regionally restricted |
3H
|
In this mutant expression is in the skin basement membrane is the same as in wild-type.
|
| TS22: epidermis |
Present |
Regionally restricted |
3H
|
In this mutant expression is in the skin basement membrane is the same as in wild-type.
|
Specimen
4H:
embryonic day 18.5
(more )
Note: Double labeled: red - Agrn; green - Pecam1 (assay MGI:7377703).
Specimen
4H:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Agrn; green - Pecam1 (assay MGI:7377703).
|
Specimen
4I:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * NMRI
|
|
Mutant Allele(s):
|
Fras1tm1Chpk/Fras1tm1Chpk
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Agrn; green - Pecam1 (assay MGI:7377703).
|
|
Analysis Tools