Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:137010
Petrou P, et al., Basement membrane distortions impair lung lobation and capillary organization in the mouse model for fraser syndrome. J Biol Chem. 2005 Mar 18;280(11):10350-6
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7377695
|
|
Gene symbol:
|
Fras1
|
|
Gene name:
|
Fraser extracellular matrix complex subunit 1
|
|
Antibody:
|
anti-Fras1
|
|
Detection system:
|
colloidal gold
|
|
Assay notes:
|
Ultrastructural immunolocalization. Specimens were fixed in 2% glutaraldehyde, 2% PFA, post-fixed in 2% aqueous OsO4 and embedded in a modified Mollenhauer's resin.
|
|
|
Results
|
Specimen
1E:
embryonic day 14.5
(more  )
Specimen
1E:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Resin/plastic
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Present |
Regionally restricted |
1E
|
Expression is underneath the lamina densa of the basement membrane zone of a terminal airway.
|
| TS22: lung epithelium |
Present |
Regionally restricted |
1E
|
Expression is underneath the lamina densa of the basement membrane zone of a terminal airway.
|
Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Resin/plastic
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Present |
Regionally restricted |
|
Expression is underneath the lamina densa of the basement membrane zone of the mesothelial monolayer.
|
| TS22: visceral pleura |
Present |
Regionally restricted |
|
Expression is underneath the lamina densa of the basement membrane zone of the mesothelial monolayer.
|
|
Analysis Tools