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Gene Expression Data
Assay Details
Assay
Reference: J:235626 Sun J, et al., Msx1 and Msx2 function together in the regulation of primordial germ cell migration in the mouse. Dev Biol. 2016 Sep 1;417(1):11-24
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7356670
Gene symbol: Fut4
Gene name: fucosyltransferase 4
Antibody: anti-SSEA-1
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 3N: embryonic day 11.5; Msx1tm1Rilm/Msx1+ (more )
Structure Level Pattern Image Note
TS19: tail Ambiguous 3N No primordial germ cells were left in the very end of the tail.
Specimen 3P: embryonic day 11.5; Msx1tm1Rilm/Msx1tm1Rilm, Msx2tm1Rilm/Msx2tm1Rilm (more )
Structure Level Pattern Image Note
TS19: primordial germ cell Present Single cells 3P Ectopic primordial germ cells found in the tail and epithelium were not incorporated into the hindgut.
TS19: tail Present Single cells 3P Ectopic primordial germ cells found in the tail and epithelium were not incorporated into the hindgut.
Specimen 4E: embryonic day 11.5; Msx1tm1Rilm/Msx1+ (more )
Note: Double labeled: green - Fut4; red - anti-H3 antibody.
Structure Level Pattern Image Note
TS19: primordial germ cell Present 4E
TS19: urogenital ridge Present 4E
Specimen 4F: embryonic day 11.5; Msx1tm1Rilm/Msx1tm1Rilm, Msx2tm1Rilm/Msx2tm1Rilm (more )
Note: Double labeled: green - Fut4; red - anti-H3 antibody.
Structure Level Pattern Image Note
TS19: primordial germ cell Present 4F An increase in mitotic activity among primordial germ cells was seen.
TS19: urogenital ridge Present 4F An increase in mitotic activity among primordial germ cells was seen.
Specimen 7C: embryonic day 9.5; Msx1tm1Rilm/Msx1+ (more )
Note: Double labeled: green - Fut4; red - Fn1 (assay MGI:7356683).
Structure Level Pattern Image Note
TS15: primordial germ cell of hindgut Present 7C
Specimen 7D: embryonic day 9.5; Msx1tm1Rilm/Msx1tm1Rilm, Msx2tm1Rilm/Msx2tm1Rilm (more )
Note: Double labeled: green - Fut4; red - Fn1 (assay MGI:7356683).
Structure Level Pattern Image Note
TS15: primordial germ cell of hindgut Present 7D
Specimen 7G'/G": embryonic day 11.5; Msx1tm1Rilm/Msx1+ (more )
Note: Double labeled: green - Fut4; red - Fn1 (assay MGI:7356683).
Structure Level Pattern Image Note
TS19: primordial germ cell Present 7G" , 7G'
Specimen 7H'/H": embryonic day 11.5; Msx1tm1Rilm/Msx1tm1Rilm, Msx2tm1Rilm/Msx2tm1Rilm (more )
Note: Double labeled: green - Fut4; red - Fn1 (assay MGI:7356683).
Structure Level Pattern Image Note
TS19: primordial germ cell Present 7H" , 7H'
Specimen S3K/K': embryonic day 11.5; Msx1tm1Rilm/Msx1tm1Rilm, Msx2tm1Rilm/Msx2tm1Rilm (more )
Note: Double labeled: green - Fut4; red - anti-H3 antibody.
Structure Level Pattern Image Note
TS19: primordial germ cell Present S3K' , S3K Ectopic primordial germ cells in the tail were mitotic.
TS19: tail Present S3K' , S3K Ectopic primordial germ cells in the tail were mitotic.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory