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Gene Expression Data
Assay Details
Assay
Reference: J:235626 Sun J, et al., Msx1 and Msx2 function together in the regulation of primordial germ cell migration in the mouse. Dev Biol. 2016 Sep 1;417(1):11-24
Assay type: RNA in situ
MGI Accession ID: MGI:7356664
Gene symbol: Msx2
Gene name: msh homeobox 2
Probe: Msx2 cDNA
Probe preparation: Antisense labelled with fluorescein RNA
Visualized with: Fluorescence
Results
Specimen 1G'/G": embryonic day 6.5 (more )
Note: Double labeled: green - Msx2; red - Msx1 (assay MGI:7356663).
Structure Level Pattern Image Note
TS9: embryo mesoderm Present 1G' , 1G"
Specimen 1H'/H": embryonic day 7.5 (more )
Note: Double labeled: green - Msx2; red - Msx1 (assay MGI:7356663).
Structure Level Pattern Image Note
TS11: allantois Strong 1H" , 1H'
TS11: embryo mesoderm Strong 1H" , 1H'
TS11: amnion mesoderm Present 1H" , 1H'
TS11: chorion mesoderm Present Regionally restricted 1H' , 1H" Expressed in some chorionic mesoderm.
Specimen 1I'/I": embryonic day 7.75 (more )
Note: Double labeled: green - Msx2; red - Msx1 (assay MGI:7356663).
Structure Level Pattern Image Note
TS12: allantois Strong 1I' , 1I"
TS12: embryo mesoderm Strong 1I' , 1I"
Specimen 2C/C': embryonic day 6.5 (more )
Note: Double labeled: green - Msx2; red - Prdm1 (assay MGI:7356666).
Structure Level Pattern Image Note
TS9: embryo mesoderm Present 2C' , 2C Expression was more concentrated in areas surrounding primordial germ cell precursors.
TS9: primitive endoderm Present 2C' , 2C Expression was more concentrated in areas surrounding primordial germ cell precursors.
TS9: primordial germ cell Present Punctate 2C , 2C' Coexpressed with Prdm1 in a subpopulation of precursors.
Specimen 2D/D': embryonic day 7.75 (more )
Note: Double labeled: green - Msx2; red - Prdm1 (assay MGI:7356666).
Structure Level Pattern Image Note
TS12: primordial germ cell Present Punctate 2D , 2D' Coexpressed with Prdm1 in a subpopulation of precursors.
TS12: embryo mesoderm Present 2D' , 2D Expression was more concentrated in areas surrounding primordial germ cell precursors.
TS12: primitive endoderm Present 2D' , 2D Expression was more concentrated in areas surrounding primordial germ cell precursors.
Specimen 2I: embryonic day 8.25 (more )
Note: Double labeled: green - Msx2; red - Ifitm3 (assay MGI:7356669).
Structure Level Pattern Image Note
TS12: allantois Present 2I
TS12: gut endoderm Present 2I
TS12: extraembryonic visceral endoderm Ambiguous 2I Not expressed significantly.
Specimen 2J: embryonic day 9.5 (more )
Note: Double labeled: green - Msx2; red - Ifitm3 (assay MGI:7356669).
Structure Level Pattern Image Note
TS15: surface ectoderm Present 2J Expressed in the body wall.
TS15: primordial germ cell of hindgut Absent 2J
Specimen 5C: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: hindgut diverticulum endoderm Present Regionally restricted 5C Expressed in visceral mesoderm in direct contact with primordial germ cell-containing hindgut.
TS13: hindgut diverticulum mesenchyme Present Regionally restricted 5C Expressed most intensely in somatic mesoderm.
TS13: neural tube Present Regionally restricted 5C Expressed in the most dorsal part.
Specimen S3B: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: hindgut mesentery Present S3B
TS17: urogenital ridge Present S3B
Specimen S3D: embryonic day 12.0 (more )
Structure Level Pattern Image Note
TS20: hindgut mesentery Present S3D
TS20: urogenital ridge Present S3D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory