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Gene Expression Data
Assay Details
Assay
Reference: J:236404 Minocha S, et al., Epiblast-specific loss of HCF-1 leads to failure in anterior-posterior axis specification. Dev Biol. 2016 Oct 1;418(1):75-88
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7356403
Gene symbol: Hcfc1
Gene name: host cell factor C1
Antibody: anti-HCF
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2B: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryonic visceral endoderm Present 2B5/6 , 2B3/4 Similar as in the control.
TS9: extraembryonic ectoderm Present 2B5/6 , 2B3/4 Similar as in the control.
TS9: extraembryonic visceral endoderm Present 2B5/6 , 2B3/4 Similar as in the control.
TS9: embryo ectoderm Weak 2B5/6 , 2B3/4 87% of epiblast cells were negative.
Specimen 2D: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: extraembryonic ectoderm Present 2D3/4 , 2D5/6 Similar, albeit less than in the control.
TS11: embryo ectoderm Weak 2D5/6 , 2D3/4 85-90% of epiblast cells were negative.
TS11: embryonic visceral endoderm Present 2D5/6 , 2D3/4 Cells failed to grow and did not show the transition from a cuboidal to a simple squamous epithelial shape.
Specimen 4A1: embryonic day 6.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 4A1
TS9: embryonic visceral endoderm Present 4A1
TS9: extraembryonic ectoderm Present 4A1
TS9: extraembryonic visceral endoderm Present 4A1
Specimen 4B1: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryonic visceral endoderm Present 4B1
TS9: extraembryonic ectoderm Present 4B1
TS9: extraembryonic visceral endoderm Present 4B1
TS9: embryo ectoderm Weak 4B1 87% of epiblast cells were negative.
Specimen 4C1: embryonic day 7.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 4C1
TS11: embryonic visceral endoderm Present 4C1
TS11: extraembryonic ectoderm Present 4C1
TS11: extraembryonic visceral endoderm Present 4C1
Specimen 4D1: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: embryo ectoderm Weak 4D1 85-90% of epiblast cells were negative.
TS11: embryonic visceral endoderm Present 4D1
TS11: extraembryonic ectoderm Present 4D1
TS11: extraembryonic visceral endoderm Present 4D1
Specimen 5A1: embryonic day 6.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 5A1
TS9: embryonic visceral endoderm Present 5A1
TS9: extraembryonic ectoderm Present 5A1
TS9: extraembryonic visceral endoderm Present 5A1
Specimen 5B1: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryo ectoderm Weak 5B1 87% of epiblast cells were negative.
TS9: embryonic visceral endoderm Present 5B1
TS9: extraembryonic ectoderm Present 5B1
TS9: extraembryonic visceral endoderm Present 5B1
Specimen 5C1: embryonic day 7.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 5C1
TS11: embryonic visceral endoderm Present 5C1
TS11: extraembryonic ectoderm Present 5C1
TS11: extraembryonic visceral endoderm Present 5C1
Specimen 5D1: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: embryo ectoderm Weak 5D1 85-90% of epiblast cells were negative.
TS11: embryonic visceral endoderm Present 5D1
TS11: extraembryonic ectoderm Present 5D1
TS11: extraembryonic visceral endoderm Present 5D1
Specimen 8A1: embryonic day 6.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 8A1
TS9: embryonic visceral endoderm Present 8A1
TS9: extraembryonic ectoderm Present 8A1
TS9: extraembryonic visceral endoderm Present 8A1
Specimen 8B1: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryonic visceral endoderm Present 8B1
TS9: extraembryonic ectoderm Present 8B1
TS9: extraembryonic visceral endoderm Present 8B1
TS9: embryo ectoderm Weak 8B1
Specimen 8C1: embryonic day 7.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 8C1
TS11: embryonic visceral endoderm Present 8C1
TS11: extraembryonic ectoderm Present 8C1
TS11: extraembryonic visceral endoderm Present 8C1
Specimen 8D1: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: embryonic visceral endoderm Present 8D1
TS11: extraembryonic ectoderm Present 8D1
TS11: extraembryonic visceral endoderm Present 8D1
TS11: embryo ectoderm Weak 8D1
Specimen S1B: embryonic day 8.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS13: embryo endoderm Present S1B3/4 , S1B2 Expressed in the visceral endoderm.
TS13: embryo ectoderm Weak S1B3/4 , S1B2 The size of mutant epiblasts also did not noticeably increase.
TS13: extraembryonic ectoderm Present S1B2 , S1B3/4 Similar, albeit less than in the control.
Specimen S1D: embryonic day 9.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: embryo Ambiguous S1D3/4 , S1D2 Mutant embryos were largely deformed and the epiblast region could not be identified easily.
TS15: embryo endoderm Present S1D3/4 , S1D2
TS15: extraembryonic ectoderm Present S1D3/4 , S1D2
Specimen S4A1: embryonic day 5.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS7: embryonic visceral endoderm Present S4A1
TS7: epiblast Present S4A1
TS7: extraembryonic ectoderm Present S4A1
TS7: extraembryonic visceral endoderm Present S4A1
Specimen S4B1: embryonic day 5.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS7: embryonic visceral endoderm Present S4B1
TS7: extraembryonic ectoderm Present S4B1
TS7: extraembryonic visceral endoderm Present S4B1
TS7: epiblast Weak S4B1
Specimen S5A1: embryonic day 8.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS13: embryo Present S5A1
TS13: extraembryonic component Present S5A1
Specimen S5B1: embryonic day 8.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS13: extraembryonic component Present S5B1
TS13: embryo Present Regionally restricted S5B1 Positive embryonic visceral endoderm was similar as in earlier embryonic stages.
TS13: embryo ectoderm Weak S5B1
Specimen S6A1: embryonic day 6.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present S6A1
TS9: embryonic visceral endoderm Present S6A1
TS9: extraembryonic ectoderm Present S6A1
TS9: extraembryonic visceral endoderm Present S6A1
Specimen S6B1: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryonic visceral endoderm Present S6B1
TS9: extraembryonic ectoderm Present S6B1
TS9: extraembryonic visceral endoderm Present S6B1
TS9: embryo ectoderm Weak S6B1
Specimen S6C1: embryonic day 7.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present S6C1
TS11: embryonic visceral endoderm Present S6C1
TS11: extraembryonic ectoderm Present S6C1
TS11: extraembryonic visceral endoderm Present S6C1
Specimen S6D1: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: embryonic visceral endoderm Present S6D1
TS11: extraembryonic ectoderm Present S6D1
TS11: extraembryonic visceral endoderm Present S6D1
TS11: embryo ectoderm Weak S6D1
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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