About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:236404 Minocha S, et al., Epiblast-specific loss of HCF-1 leads to failure in anterior-posterior axis specification. Dev Biol. 2016 Oct 1;418(1):75-88
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7356403
Gene symbol: Hcfc1
Gene name: host cell factor C1
Antibody: anti-HCF
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2B: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryonic visceral endoderm Present 2B5/6 , 2B3/4 Similar as in the control.
TS9: extraembryonic ectoderm Present 2B5/6 , 2B3/4 Similar as in the control.
TS9: extraembryonic visceral endoderm Present 2B5/6 , 2B3/4 Similar as in the control.
TS9: embryo ectoderm Weak 2B5/6 , 2B3/4 87% of epiblast cells were negative.
Specimen 2D: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: extraembryonic ectoderm Present 2D3/4 , 2D5/6 Similar, albeit less than in the control.
TS11: embryo ectoderm Weak 2D5/6 , 2D3/4 85-90% of epiblast cells were negative.
TS11: embryonic visceral endoderm Present 2D5/6 , 2D3/4 Cells failed to grow and did not show the transition from a cuboidal to a simple squamous epithelial shape.
Specimen 4A1: embryonic day 6.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 4A1
TS9: embryonic visceral endoderm Present 4A1
TS9: extraembryonic ectoderm Present 4A1
TS9: extraembryonic visceral endoderm Present 4A1
Specimen 4B1: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryonic visceral endoderm Present 4B1
TS9: extraembryonic ectoderm Present 4B1
TS9: extraembryonic visceral endoderm Present 4B1
TS9: embryo ectoderm Weak 4B1 87% of epiblast cells were negative.
Specimen 4C1: embryonic day 7.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 4C1
TS11: embryonic visceral endoderm Present 4C1
TS11: extraembryonic ectoderm Present 4C1
TS11: extraembryonic visceral endoderm Present 4C1
Specimen 4D1: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: embryo ectoderm Weak 4D1 85-90% of epiblast cells were negative.
TS11: embryonic visceral endoderm Present 4D1
TS11: extraembryonic ectoderm Present 4D1
TS11: extraembryonic visceral endoderm Present 4D1
Specimen 5A1: embryonic day 6.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 5A1
TS9: embryonic visceral endoderm Present 5A1
TS9: extraembryonic ectoderm Present 5A1
TS9: extraembryonic visceral endoderm Present 5A1
Specimen 5B1: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryo ectoderm Weak 5B1 87% of epiblast cells were negative.
TS9: embryonic visceral endoderm Present 5B1
TS9: extraembryonic ectoderm Present 5B1
TS9: extraembryonic visceral endoderm Present 5B1
Specimen 5C1: embryonic day 7.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 5C1
TS11: embryonic visceral endoderm Present 5C1
TS11: extraembryonic ectoderm Present 5C1
TS11: extraembryonic visceral endoderm Present 5C1
Specimen 5D1: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: embryo ectoderm Weak 5D1 85-90% of epiblast cells were negative.
TS11: embryonic visceral endoderm Present 5D1
TS11: extraembryonic ectoderm Present 5D1
TS11: extraembryonic visceral endoderm Present 5D1
Specimen 8A1: embryonic day 6.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 8A1
TS9: embryonic visceral endoderm Present 8A1
TS9: extraembryonic ectoderm Present 8A1
TS9: extraembryonic visceral endoderm Present 8A1
Specimen 8B1: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryonic visceral endoderm Present 8B1
TS9: extraembryonic ectoderm Present 8B1
TS9: extraembryonic visceral endoderm Present 8B1
TS9: embryo ectoderm Weak 8B1
Specimen 8C1: embryonic day 7.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 8C1
TS11: embryonic visceral endoderm Present 8C1
TS11: extraembryonic ectoderm Present 8C1
TS11: extraembryonic visceral endoderm Present 8C1
Specimen 8D1: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: embryonic visceral endoderm Present 8D1
TS11: extraembryonic ectoderm Present 8D1
TS11: extraembryonic visceral endoderm Present 8D1
TS11: embryo ectoderm Weak 8D1
Specimen S1B: embryonic day 8.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS13: embryo endoderm Present S1B3/4 , S1B2 Expressed in the visceral endoderm.
TS13: embryo ectoderm Weak S1B3/4 , S1B2 The size of mutant epiblasts also did not noticeably increase.
TS13: extraembryonic ectoderm Present S1B2 , S1B3/4 Similar, albeit less than in the control.
Specimen S1D: embryonic day 9.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: embryo Ambiguous S1D3/4 , S1D2 Mutant embryos were largely deformed and the epiblast region could not be identified easily.
TS15: embryo endoderm Present S1D3/4 , S1D2
TS15: extraembryonic ectoderm Present S1D3/4 , S1D2
Specimen S4A1: embryonic day 5.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS7: embryonic visceral endoderm Present S4A1
TS7: epiblast Present S4A1
TS7: extraembryonic ectoderm Present S4A1
TS7: extraembryonic visceral endoderm Present S4A1
Specimen S4B1: embryonic day 5.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS7: embryonic visceral endoderm Present S4B1
TS7: extraembryonic ectoderm Present S4B1
TS7: extraembryonic visceral endoderm Present S4B1
TS7: epiblast Weak S4B1
Specimen S5A1: embryonic day 8.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS13: embryo Present S5A1
TS13: extraembryonic component Present S5A1
Specimen S5B1: embryonic day 8.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS13: extraembryonic component Present S5B1
TS13: embryo Present Regionally restricted S5B1 Positive embryonic visceral endoderm was similar as in earlier embryonic stages.
TS13: embryo ectoderm Weak S5B1
Specimen S6A1: embryonic day 6.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present S6A1
TS9: embryonic visceral endoderm Present S6A1
TS9: extraembryonic ectoderm Present S6A1
TS9: extraembryonic visceral endoderm Present S6A1
Specimen S6B1: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: embryonic visceral endoderm Present S6B1
TS9: extraembryonic ectoderm Present S6B1
TS9: extraembryonic visceral endoderm Present S6B1
TS9: embryo ectoderm Weak S6B1
Specimen S6C1: embryonic day 7.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present S6C1
TS11: embryonic visceral endoderm Present S6C1
TS11: extraembryonic ectoderm Present S6C1
TS11: extraembryonic visceral endoderm Present S6C1
Specimen S6D1: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: embryonic visceral endoderm Present S6D1
TS11: extraembryonic ectoderm Present S6D1
TS11: extraembryonic visceral endoderm Present S6D1
TS11: embryo ectoderm Weak S6D1
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory