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Gene Expression Data
Assay Details
Assay
Reference: J:306806 Hauser BR, et al., Generation of a Single-Cell RNAseq Atlas of Murine Salivary Gland Development. iScience. 2020 Dec 18;23(12):101838
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7356287
Gene symbol: Bhlha15
Gene name: basic helix-loop-helix family, member a15
Antibody: anti-MIST1/bHLHa15 (D7N4B) (14896)
Detection system: Secondary antibody
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-MIST1 MGI:7356043 may have been used in this assay.
Results
Specimen 4C P30 female: postnatal day 30 (more )
Note: Triple labeled: white - Bhlha15 (Mist1); green - Lpo (assay MGI:7356280); red - Prol1 (assay MGI:7356285).
Structure Level Pattern Image Note
TS28: submandibular gland Present Regionally restricted 4C P30 female Expression suggests seromucous cell type.
Specimen 4C P30 male: postnatal day 30 (more )
Note: Triple labeled: white - Bhlha15 (Mist1); green - Lpo (assay MGI:7356280); red - Prol1 (assay MGI:7356285).
Structure Level Pattern Image Note
TS28: submandibular gland Present Regionally restricted 4C P30 male Expression suggests seromucous cell type.
Specimen 8A P1 (upper): postnatal day 1 (more )
Note: Double labeled: red - Bhlha15 (Mist1); green - Smgc (assay MGI:7356290).
Structure Cell Type Level Pattern Image Note
TS27: submandibular gland acinar cell of salivary gland Present Regionally restricted 8A P1 (upper) Co-expression with Smgc-expressing cells.
Specimen 8A P30 female (upper): postnatal day 30 (more )
Note: Double labeled: red - Bhlha15 (Mist1); green - Smgc (assay MGI:7356290).
Structure Cell Type Level Pattern Image Note
TS28: submandibular gland acinar cell of salivary gland Present 8A P30 female (upper)
Specimen 8A P30 male (upper): postnatal day 30 (more )
Note: Double labeled: red - Bhlha15 (Mist1); green - Smgc (assay MGI:7356290).
Structure Cell Type Level Pattern Image Note
TS28: submandibular gland acinar cell of salivary gland Present 8A P30 male (upper)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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