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Gene Expression Data
Assay Details
Assay
Reference: J:200761 Kasberg AD, et al., SP8 regulates signaling centers during craniofacial development. Dev Biol. 2013 Sep 15;381(2):312-23
Assay type: RT-PCR
MGI Accession ID: MGI:7356013
Gene symbol: Smo
Gene name: smoothened, frizzled class receptor
Probe: Smo-pQ, Smo-pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Actb or Tubb5. There were two sets of primers in the paper. It was not specified which one was used.
Results
Image: S5F Smo ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
E8.5 WT E8.5 TS13: anterior neural ridge
TS13: facial neural crest
TS13: neural fold 		Present Not Specified; total RNA Not Specified Not Specified cranial neural folds
E8.5 Sp8-/- E8.5 TS13: anterior neural ridge
TS13: facial neural crest
TS13: neural fold 		Present (a) Not Specified; total RNA involves: 129S1/Sv * 129X1/SvJ Sp8tm1Smb/Sp8tm1SmbNot Specified cranial neural folds
E9.5 WT E9.5 TS15: face
TS15: neural plate 		Present Not Specified; total RNA Not Specified Not Specified middle to anterior facial structures including the anterior neural ridge
E9.5 Sp8-/- E9.5 TS15: face
TS15: neural plate 		Present (a) Not Specified; total RNA involves: 129S1/Sv * 129X1/SvJ Sp8tm1Smb/Sp8tm1SmbNot Specified middle to anterior facial structures including the anterior neural ridge
- Other lane(s) in blot assay different gene(s)
Notes:
(a) Similar as in the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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